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Research Technologies Branch |
Biological Imaging Section
Technology: Instrument Specifications
Instrument |
Configuration and Capabilities |
| Leica TCS-NT/SP1 Confocal Microscope |
4 lasers: UV-Ar, Ar, Kr, HeNe
7 excitation wavelengths: 364, 457, 476, 488, 514, 568, 633nm
Detection: 4 fluorescence channels + transmitted light
Microscope: Leica DMIRBE equipped with 10, 20, 40, 63, and 100x DIC objectives, 40 Ph2 objective
Software: Leica TCS v 2.1227 |
| Leica SP2-AOBS/MP Confocal Microscope |
6 lasers: UV-Ar, Ar, Kr, HeNe, GrHeNe, OrHeNe
Multiphoton laser: Spectra Physics Wideband Mai-Tai
8 excitation wavelengths: 364, 457, 476, 488, 514, 594, 543, 633nm
Detection: 4 fluorescence channels + transmitted light
Microscope: Leica DMIRBE equipped with 10, 20, 40, 63, 100x DIC objectives
Software: Leica TCS v. 2.1227 |
| Leica DMIRBE Inverted Fluorescence Microscope (aka "video-microscope") |
Objectives: 10x, 20xLWD, 40xLWD, 40x oil, 63x oil, 100x DIC,
10x, 20x, 40x Phase
Illumination: Transmitted light - 100W halogen light
Epi-illumination - 100W Hg lamp
Fluorescence filters: Dapi, FITC, TR also available: Cy5, Cy3
Cameras: Cooke Sensicam QE 1376 x 1040 cooled CCD
Hamamatsu C5985 video rate CCD (30fps)
Hamamatsu C2400 intensified CD (30fps, low light)
Additional: Marzhauser x-y stage, illumination shutters
Software: Media Cybernetics Image Pro Plus 4.5 |
| Leica DMIRBE Inverted Fluorescence Microscope |
Objectives: 10x, 20xLWD, 40xLWD, 40x oil, 63x oil, 100x oil
all objectives equipped for DIC
Illumination: Transmitted light - 100W halogen light
Epi-illumination - 100W Hg lamp
Fluorescence filters: Dapi, FITC, TR also available: Cy5, Cy3
Cameras: Leica SC150, Zeiss Axiocam Color, Leica film camera
Discussion tube for two simultaneous users |
| Zeiss Axiovert 100 |
Objectives: 5xLWD, 20xLWD Ph2, 40xLWD Ph2
Illumination: Transmitted light - 100W halogen light
Cameras: 35mm film, Zeiss Axiocam Color, Leica DC 150 |
| Leica MZ 9.5 Dissection Microscope |
Magnification range: 6.3x–60x
Illumination: Transmitted light, Darkfield, Incident light, Epifluorescence (488nm for GFP)
Cameras: 35mm film, Zeiss Axiocam Color, or Leica DC150 |
| Microinjection Equipment |
3 Eppendorf 3 axis micromanipulators (model 5171)
1 Eppendorf InjectMan microinjection controller (5246)
2 Eppendorf TransferMan pressure systems
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| Workstations |
3 Dual Xeon workstations
1 SGI 540 Quad processor workstation |
| Scanners |
HP flatbed scanner
Nikon Coolscan 2000 (35mm scanner) |
| Temperature Controlled Stages |
2 Bioptechs Delta T systems with heated lid
Harvard Instruments controller
Harvard Instruments CSMI rectangular heated stage
Harvard Instruments PDMI2 circular heated stage
20/20 Technologies Bionomic controller and BC300 stage
Pe-Con Environmental Chamber (Incubator S, Heating Insert P) with CO2 Controller |
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