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Title Modulation of 2{prime}-5{prime} oligoadenylate synthetase by environmental stress in the marine sponge Geodia cydonium
Creator/Author Schroeder, H.C. ; Wiens, M. ; Mueller, W.E.G. [Abteilung Angewandte Molekularbiologie, Mainz (Germany). Inst. fuer Physiologische Chemie] ; Kuusksalu, A. ; Kelve, M. [Inst. of Chemical Physics and Biophysics, Tallinn (Estonia)]
Publication Date1997 Jul 01
OSTI IdentifierOSTI ID: 514571
Other Number(s)ETOCDK; ISSN 0730-7268
Resource TypeJournal Article
Resource RelationEnvironmental Toxicology and Chemistry ; VOL. 16 ; ISSUE: 7 ; PBD: Jul 1997
Subject54 ENVIRONMENTAL SCIENCES ;56 BIOLOGY AND MEDICINE, APPLIED STUDIES ; BIOLOGICAL INDICATORS; WATER POLLUTION; BIOLOGICAL MARKERS; LIGASES; BIOLOGICAL STRESS; INVERTEBRATES; AQUATIC ORGANISMS; ENZYME ACTIVITY
Description/Abstract Recently the authors established the presence of relatively high amounts of 2{prime}-5{prime} oligoadenylates (2{prime}-5{prime} A) and 2{prime}-5{prime} oligoadenylate synthetase (2{prime}-5{prime} A synthetase) in the marine sponge Geodia cydonium. Here they determined by applying radioimmunoassay and high-performance liquid chromatographical methods that the concentration of 2{prime}-5{prime} A synthetase change following exposure of G. cydonium tissue to environmental stress. The 2{prime}-5{prime} A content and the activity of 2{prime}-5{prime} A synthetase, present in crude sponge extract, increase by up to three-fold after treating sponge cubes for 2 h with natural stressors including heat shock (26 C), cold shock (6 C), pH shock (pH 6), and hypertonic shock and subsequent incubation for 18 h under ambient conditions (16 C). No response was observed after exposure of sponges to an alkaline (pH 10) or hypotonic environment. Similar changes have been found for the expression of heat shock protein HSP70 in G. cydonium. These results show that 2{prime}-5{prime} A in sponges may be useful as a novel biomarker for environmental monitoring.
Country of PublicationUnited States
LanguageEnglish
Formatpp. 1403-1409 ; PL:
System Entry Date2001 May 05

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