Protein synthesis of the sponge Geodia cydonium: Characterization of the system

Nucleic Acids Res. 1974 June; 1(6): 835–847.

PMCID: PMC343384

Werner E.G. Müller, Isabel Müller, and Rudolf K. Zahn

Physiologisch-Chemisches Institut der Johannes Gutenberg Universität, 65 Mainz, Johann Joachim Becher Weg 13, West Germany

Abstract

The ribosomal population of the sponge Geodia cydonium has been examined. The monosomes have a sedimentation constant of 80 S, the sizes of the subunits are approximately 60 S and 45 S respectively. The polyribosomes contain up to 40 ribosomal units. Cell free protein synthesizing systems (cell homogenate as well as reconstituted system) have been prepared and characterized with respect to Mg²⁺, KCI and ATP concentrations, temperature, pH and time course of the reaction. In the cell-free system and in the cellular system the protein biosynthesis is inhibited by chloramphenicol. It is not affected by cycloheximide.

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