WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.
BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.
BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract
Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract
RepeatMasker Server unavailable.Reference: Gish, Warren (1994-1997). unpublished. Gish, Warren and David J. States (1993). Identification of protein coding regions by database similarity search. Nat. Genet. 3:266-72.Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.
Query= B09B08.seq(1>517) (488 letters)
Translating both strands of query sequence in all 6 reading framesDatabase: nr 625,274 sequences; 197,782,623 total letters.Observed Numbers of Database Sequences Satisfying Various EXPECTation Thresholds (E parameter values) Histogram units: = 7 Sequences : less than 7 sequences EXPECTation Threshold (E parameter) | V Observed Counts--> 10000 1771 381 |====================================================== 6310 1390 260 |===================================== 3980 1130 230 |================================ 2510 900 295 |========================================== 1580 605 159 |====================== 1000 446 91 |============= 631 355 79 |=========== 398 276 61 |======== 251 215 57 |======== 158 158 34 |==== 100 124 12 |= 63.1 112 22 |=== 39.8 90 9 |= 25.1 81 12 |= 15.8 69 3 |: >>>>>>>>>>>>>>>>>>>>> Expect = 10.0, Observed = 66 <<<<<<<<<<<<<<<<< 10.0 66 3 |: 6.31 63 6 |: 3.98 57 2 |: 2.51 55 3 |: 1.58 52 2 |: 1.00 50 1 |: 0.63 49 1 |: 0.40 48 4 |: 0.25 44 2 |: 0.16 42 1 |: 0.10 41 0 | 0.063 41 0 | 0.040 41 0 | 0.025 41 2 |: 0.016 39 3 |: 0.010 36 7 |= 0.0063 29 0 | 0.0040 29 1 |: Smallest Sum Reading High Probability Sequences producing High-scoring Segment Pairs: Frame Score P(N) N gi|2435395|gb|AAB71208.1|(U63550) pectate lyase [Frag... +3 264 1.1e-21 1 gi|9279795|dbj|BAB01216.1|(AP000740) pectate lyase [A... +3 261 4.6e-21 1 gi|2494113|gb|AAB80622.1|(AC002376) Strong similarity... +3 254 2.3e-20 1 gi|6729008|gb|AAF27005.1|AC016827_16(AC016827) putati... +3 245 2.0e-19 1 gi|11994243|dbj|BAB01365.1|(AB028621) pectate lyase [... +3 243 4.8e-19 1 gi|7449068|pir||T07701pectate lyase (EC 4.2.2.2) F17N... +3 232 5.9e-18 1 gi|10177179|dbj|BAB10313.1|(AB017061) pectate lyase [... +3 230 7.6e-18 1 gi|6606534|gb|AAF19196.1|AF206320_1(AF206320) pectate... +3 229 1.7e-17 1 gi|10177299|dbj|BAB10560.1|(AB008265) pectate lyase [... +3 213 7.2e-16 1 gi|7547009|gb|AAF63756.1|AF243475_1(AF243475) pectate... +3 205 5.0e-15 1 gi|7449066|pir||T05240pectate lyase (EC 4.2.2.2) A11 ... +3 203 6.7e-15 1 gi|1256509|emb|CAA63496.1|(X92943) pectate lyase [Mus... +3 199 2.3e-14 1 gi|6606532|gb|AAF19195.1|AF206319_1(AF206319) pectate... +3 199 2.5e-14 1 gi|7449067|pir||T05556pectate lyase (EC 4.2.2.2) F22K... +3 192 1.4e-13 1 gi|2463509|emb|CAA70735.1|(Y09541) pectate lyase [Zin... +3 190 2.3e-13 1 gi|11072028|gb|AAG28907.1|AC008113_23(AC008113) F12A2... +3 172 2.1e-11 1 gi|7939512|dbj|BAA95715.1|(AB024028) pectate lyase-li... +3 171 3.0e-11 1 gi|7449062|pir||T03932pectate lyase (EC 4.2.2.2) Nt59... +3 156 4.0e-10 1 gi|11279704|pir||T46165pectate lyase-like protein - A... +3 139 1.1e-07 1 gi|7449065|pir||T06728pectate lyase (EC 4.2.2.2) F28P... +3 123 1.4e-07 2 gi|629849|pir||S43335pectate lyase (EC 4.2.2.2) - mai... +3 129 1.2e-06 1 gi|125889|sp|P15722|PE59_LYCESPROBABLE PECTATE LYASE ... +3 124 4.5e-06 1 gi|9758606|dbj|BAB09239.1|(AB009050) pectate lyase [A... +3 120 9.8e-06 1 gi|6094557|gb|AAF03499.1|AC010676_9(AC010676) putativ... +3 119 1.7e-05 1 gi|730290|sp|P40973|PEL_LILLOPECTATE LYASE PRECURSOR ... +3 117 4.9e-05 1 gi|112863|sp|P24396|9612_LYCESSTYLE DEVELOPMENT-SPECI... +3 102 0.00062 2 gi|2281334|gb|AAB69759.1|(U83619) putative pectate ly... +3 112 0.00083 1 gi|7262684|gb|AAF43942.1|AC012188_19(AC012188) Strong... +3 112 0.00083 1 gi|730291|sp|P40972|PEL_TOBACPECTATE LYASE PRECURSOR ... +3 108 0.0028 1 gi|9087152|sp|P81294|MPA1_JUNASMAJOR POLLEN ALLERGEN ... +3 104 0.0095 1 gi|8101711|gb|AAF72625.1|AF257491_1(AF257491) Cup s 1... +3 104 0.0095 1 gi|8101713|gb|AAF72626.1|AF257492_1(AF257492) Cup s 1... +3 104 0.0095 1 gi|8101715|gb|AAF72627.1|AF257493_1(AF257493) Cup s 1... +3 104 0.0095 1 gi|8101717|gb|AAF72628.1|AF257494_1(AF257494) Cup s 1... +3 104 0.0095 1 gi|8101719|gb|AAF72629.1|AF257495_1(AF257495) Cup s 1... +3 104 0.0095 1 gi|9087163|sp|Q96385|MPA1_CHAOBMAJOR POLLEN ALLERGEN ... +3 104 0.0098 1 gi|1173367|sp|P18632|SBP_CRYJASUGI BASIC PROTEIN PREC... +3 103 0.013 1 gi|541802|pir||JC2124major allergen Cry j I precursor... +3 103 0.013 1 gi|516728|dbj|BAA07020.1|(D34639) Cry j I precursor [... +3 103 0.013 1 gi|8843917|gb|AAF80164.1|(AF151427) pollen major alle... +3 102 0.017 1 gi|8843921|gb|AAF80166.1|(AF151429) pollen major alle... +3 102 0.017 1 gi|11358602|pir||T51456pectate lyase-like protein - A... +3 96 0.14 1 gi|9087167|sp|Q9SCG9|MPA1_CUPARMAJOR POLLEN ALLERGEN ... +3 94 0.16 1 gi|113478|sp|P28744|MP14_AMBARPOLLEN ALLERGEN AMB A 1... +3 94 0.19 1 gi|539051|pir||E53240allergen Amb a II precursor - co... +3 93 0.25 1 gi|113476|sp|P27760|MP12_AMBARPOLLEN ALLERGEN AMB A 1... +3 93 0.25 1 gi|539048|pir||B53240allergen Amb a I.2 precursor - c... +3 93 0.25 1 gi|1352734|sp|P15721|PE56_LYCESPROBABLE PECTATE LYASE... +3 92 0.32 1 gi|7449064|pir||T00856pectate lyase (EC 4.2.2.2) T20F... +3 92 0.37 1 gi|280372|pir||A44773pollen allergen I - Japanese ced... +3 68 0.61 1
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WARNING: Descriptions of 16 database sequences were not reported due to the limiting value of parameter V = 50.>gi|2435395|gb|AAB71208.1| (U63550) pectate lyase [Fragaria x ananassa] Length = 405 Frame 3 hits (HSPs): _______________ __________________________________________________ Database sequence: | | | | 405 0 150 300 Plus Strand HSPs: Score = 264 (92.9 bits), Expect = 1.1e-21, P = 1.1e-21 Identities = 63/122 (51%), Positives = 82/122 (67%), Frame = +3 Query: 99 MAVSSSSATKWV----VFLLLALLIREEA-MATPQISDLRNLEVERHRLPSLTNSSMAER 263 M ++SS AT + LLLALL+ A + + R +EV S NSSMA+R Sbjct: 1 MRLASSIATMHFYMTPLLLLLALLVCVSASVENGKPVQSRFVEVVEEPRSSF-NSSMADR 59 Query: 264 AKEAEKLNEQAAVANPEEVVSMVEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRN 443 + + NE A V NPEE+ S+V+ SI+NS+ RR+LG+FSC TGNPIDDCWRCDP WQR+ Sbjct: 60 SND--HWNEHA-VDNPEEIASLVDTSIRNSSTRRELGYFSCATGNPIDDCWRCDPQWQRH 116 Query: 444 RKRLAD 461 RKR A+ Sbjct: 117 RKRPAN 122
>gi|9279795|dbj|BAB01216.1| (AP000740) pectate lyase [Arabidopsis thaliana] Length = 449 Frame 3 hits (HSPs): ____________ __________________________________________________ Database sequence: | | | | 449 0 150 300 Plus Strand HSPs: Score = 261 (91.9 bits), Expect = 4.6e-21, P = 4.6e-21 Identities = 56/107 (52%), Positives = 69/107 (64%), Frame = +3 Query: 141 LLLALLIREEAMATPQISDLRNLEVERHRLPSLTNSSMAERAKEAEKLNEQAAVANPEEV 320 ++LAL I MA + DL + H S +NSS A + + + AV NPEEV Sbjct: 16 IILALCINSSTMAQ-ETEDLNS-----H---SSSNSSTANKLPNDDGAWNEHAVKNPEEV 66 Query: 321 VSMVEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 +MV+M I+NSTERR+LGFFSC TGNPIDDCWRCD NW RKRLA+ Sbjct: 67 AAMVDMKIKNSTERRRLGFFSCATGNPIDDCWRCDRNWHLRRKRLAN 113
>gi|2494113|gb|AAB80622.1| (AC002376) Strong similarity to Musa pectate lyase (gb|X92943). ESTs gb|AA042458, gb|ATTS4502, gb|N38552 come from this gene. [Arabidopsis thaliana] Length = 431 Frame 3 hits (HSPs): _________ __________________________________________________ Database sequence: | | | | 431 0 150 300 Plus Strand HSPs: Score = 254 (89.4 bits), Expect = 2.3e-20, P = 2.3e-20 Identities = 48/75 (64%), Positives = 58/75 (77%), Frame = +3 Query: 243 NSSMAERAKEAEKLNEQAAVANPEEV----VSMVEMSIQNSTERRKLGFFSCGTGNPIDD 410 N S R+ E E Q AV NP+EV +++ EMS++N TERRKLG+F+CGTGNPIDD Sbjct: 28 NISSLPRSDETEW--NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDD 85 Query: 411 CWRCDPNWQRNRKRLAD 461 CWRCDPNW +NRKRLAD Sbjct: 86 CWRCDPNWHKNRKRLAD 102
>gi|6729008|gb|AAF27005.1|AC016827_16 (AC016827) putative pectate lyase [Arabidopsis thaliana] Length = 416 Frame 3 hits (HSPs): __________ __________________________________________________ Database sequence: | | | | 416 0 150 300 Plus Strand HSPs: Score = 245 (86.2 bits), Expect = 2.0e-19, P = 2.0e-19 Identities = 45/75 (60%), Positives = 55/75 (73%), Frame = +3 Query: 237 LTNSSMAERAKEAEKLNEQAAVANPEEVVSMVEMSIQNSTERRKLGFFSCGTGNPIDDCW 416 LT + A + + + AV NP+EV +MV+MSI+NSTERR+LG+FSC TGNPIDDCW Sbjct: 15 LTALFIGVNASRSNETWHEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCW 74 Query: 417 RCDPNWQRNRKRLAD 461 RCD WQ RKRLAD Sbjct: 75 RCDRKWQLRRKRLAD 89
>gi|11994243|dbj|BAB01365.1| (AB028621) pectate lyase [Arabidopsis thaliana] Length = 452 Frame 3 hits (HSPs): ______________ __________________________________________________ Database sequence: | | | || 452 0 150 300 450 Plus Strand HSPs: Score = 243 (85.5 bits), Expect = 4.8e-19, P = 4.8e-19 Identities = 57/125 (45%), Positives = 76/125 (60%), Frame = +3 Query: 99 MAVSS---SSATKWVVFLLLALLIREEAMATPQISDLRNLEVERHRLPSLTNSSMAERAK 269 MA SS +SA ++F+ + ++ + +IS R L+ E + S +S M R Sbjct: 1 MATSSLKLTSACFVLLFIFVGCVLTATNLRNNEISRSRKLKTEDSK--SFNSSPMTTRLD 58 Query: 270 EAEKLNEQAAVANPE----EVVSMVEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQ 437 +LNE A V +P+ EV +++ MS QN T RRKLGFFSCG GN IDDCWRCD NW Sbjct: 59 GVVELNEHA-VTDPDKVAHEVSNLIHMSEQNITARRKLGFFSCGNGNLIDDCWRCDRNWN 117 Query: 438 RNRKRLAD 461 +NRK LAD Sbjct: 118 KNRKHLAD 125
>gi|7449068|pir||T07701 pectate lyase (EC 4.2.2.2) F17N18.100 - Arabidopsis thaliana >gi|4753655|emb|CAB41931.1| (AL049751) pectate lyase like protein [Arabidopsis thaliana] >gi|7268023|emb|CAB78363.1| (AL161535) pectate lyase like protein [Arabidopsis thaliana] Length = 418 Frame 3 hits (HSPs): _______ __________________________________________________ Database sequence: | | | | 418 0 150 300 Plus Strand HSPs: Score = 232 (81.7 bits), Expect = 5.9e-18, P = 5.9e-18 Identities = 42/56 (75%), Positives = 47/56 (83%), Frame = +3 Query: 297 AVANPEEVVSMVEMSIQNSTERRKLGFFS-CGTGNPIDDCWRCDPNWQRNRKRLAD 461 AV +PEE+ +MV+MSI+NST RRKLGFFS C TGNPIDDCWRCD W R RKRLAD Sbjct: 36 AVKDPEEIAAMVDMSIRNSTYRRKLGFFSSCSTGNPIDDCWRCDKKWHRRRKRLAD 91
>gi|10177179|dbj|BAB10313.1| (AB017061) pectate lyase [Arabidopsis thaliana] Length = 393 Frame 3 hits (HSPs): ________ __________________________________________________ Database sequence: | | | | 393 0 150 300 Plus Strand HSPs: Score = 230 (81.0 bits), Expect = 7.6e-18, P = 7.6e-18 Identities = 40/55 (72%), Positives = 46/55 (83%), Frame = +3 Query: 297 AVANPEEVVSMVEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 AV NPEEV +MV+MSI+NST RR+LG+FSC TGNPIDDCWRCD WQ RK LA+ Sbjct: 13 AVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLAN 67
>gi|6606534|gb|AAF19196.1|AF206320_1 (AF206320) pectate lyase 2 [Musa acuminata] Length = 454 Frame 3 hits (HSPs): ______________ __________________________________________________ Database sequence: | | | || 454 0 150 300 450 Plus Strand HSPs: Score = 229 (80.6 bits), Expect = 1.7e-17, P = 1.7e-17 Identities = 53/126 (42%), Positives = 76/126 (60%), Frame = +3 Query: 114 SSATKWV---VFLLLA-LLIREEA---MATPQISDLRNLEVERHRLPSLT-NSSMAERAK 269 ++ +W+ + LLL LL+ + + + + S RN R L + N++ A+ + Sbjct: 2 TAGLRWIPPLLLLLLGFLLVLNGSRGWIGSERSSGSRNGGASRRSLREASANATSADASL 61 Query: 270 EAEKLNE--QAAVANPEEVVSMVEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRN 443 E + +AAV +PEEV S V +I NST RR LG+ SCG+GNPIDDCWRCDP+W N Sbjct: 62 EERAVTRAAEAAVDDPEEVASTVLTTIINSTARRSLGYLSCGSGNPIDDCWRCDPDWHVN 121 Query: 444 RKRLAD 461 RK+LAD Sbjct: 122 RKKLAD 127
>gi|10177299|dbj|BAB10560.1| (AB008265) pectate lyase [Arabidopsis thaliana] Length = 410 Frame 3 hits (HSPs): _______ __________________________________________________ Database sequence: | | | | 410 0 150 300 Plus Strand HSPs: Score = 213 (75.0 bits), Expect = 7.2e-16, P = 7.2e-16 Identities = 37/54 (68%), Positives = 43/54 (79%), Frame = +3 Query: 300 VANPEEVVSMVEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 V++PE VV V I S RRKLGFFSCG+GNPIDDCWRCD +W++NRKRLAD Sbjct: 28 VSDPELVVEEVHRKINESISRRKLGFFSCGSGNPIDDCWRCDKDWEKNRKRLAD 81
>gi|7547009|gb|AAF63756.1|AF243475_1 (AF243475) pectate lyase [Vitis vinifera] Length = 398 Frame 3 hits (HSPs): ________ __________________________________________________ Database sequence: | | | | 398 0 150 300 Plus Strand HSPs: Score = 205 (72.2 bits), Expect = 5.0e-15, P = 5.0e-15 Identities = 39/60 (65%), Positives = 43/60 (71%), Frame = +3 Query: 282 LNEQAAVANPEEVVSMVEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 L A V +PE VV V SI S RR LG+FSCGTGNPIDDCWRCD NW +NR+RLAD Sbjct: 14 LGSPAPVQDPEVVVQEVHRSINAS--RRNLGYFSCGTGNPIDDCWRCDANWDKNRQRLAD 71
>gi|7449066|pir||T05240 pectate lyase (EC 4.2.2.2) A11 - Arabidopsis thaliana (fragment) >gi|4455300|emb|CAB36835.1| (AL035528) putative pectate lyase A11 (fragment) [Arabidopsis thaliana] >gi|7268075|emb|CAB78413.1| (AL161537) putative pectate lyase A11 (fragment) [Arabidopsis thaliana] Length = 374 Frame 3 hits (HSPs): _______ __________________________________________________ Database sequence: | | | | 374 0 150 300 Plus Strand HSPs: Score = 203 (71.5 bits), Expect = 6.7e-15, P = 6.7e-15 Identities = 35/41 (85%), Positives = 36/41 (87%), Frame = +3 Query: 339 SIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 S QN T RRKLGFFSCGTGNPIDDCWRCD NW +NRKRLAD Sbjct: 7 SEQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKNRKRLAD 47
>gi|1256509|emb|CAA63496.1| (X92943) pectate lyase [Musa acuminata] Length = 398 Frame 3 hits (HSPs): _______ __________________________________________________ Database sequence: | | | | 398 0 150 300 Plus Strand HSPs: Score = 199 (70.1 bits), Expect = 2.3e-14, P = 2.3e-14 Identities = 37/56 (66%), Positives = 44/56 (78%), Frame = +3 Query: 294 AAVANPEEVVSMVEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 AAV +PE VV V+ S+ S RR+LG+ SCGTGNPIDDCWRCDP+W NR+RLAD Sbjct: 17 AAVRDPELVVQEVQRSLNVS--RRRLGYLSCGTGNPIDDCWRCDPDWADNRQRLAD 70
>gi|6606532|gb|AAF19195.1|AF206319_1 (AF206319) pectate lyase 1 [Musa acuminata] Length = 407 Frame 3 hits (HSPs): _______ __________________________________________________ Database sequence: | | | | 407 0 150 300 Plus Strand HSPs: Score = 199 (70.1 bits), Expect = 2.5e-14, P = 2.5e-14 Identities = 37/56 (66%), Positives = 44/56 (78%), Frame = +3 Query: 294 AAVANPEEVVSMVEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 AAV +PE VV V+ S+ S RR+LG+ SCGTGNPIDDCWRCDP+W NR+RLAD Sbjct: 27 AAVRDPELVVQEVQRSLNVS--RRRLGYLSCGTGNPIDDCWRCDPDWADNRQRLAD 80
>gi|7449067|pir||T05556 pectate lyase (EC 4.2.2.2) F22K18.20 - Arabidopsis thaliana >gi|4220512|emb|CAA22985.1| (AL035356) putative pectate lyase [Arabidopsis thaliana] >gi|7269329|emb|CAB79388.1| (AL161562) putative pectate lyase [Arabidopsis thaliana] Length = 404 Frame 3 hits (HSPs): ________ __________________________________________________ Database sequence: | | | | 404 0 150 300 Plus Strand HSPs: Score = 192 (67.6 bits), Expect = 1.4e-13, P = 1.4e-13 Identities = 35/55 (63%), Positives = 42/55 (76%), Frame = +3 Query: 300 VANPEEVVSMVEMSIQNSTE-RRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 V +PE VV V SI S RRKLG+ SC TGNPIDDCWRCDP+W+++R+RLAD Sbjct: 25 VPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCWRCDPHWEQHRQRLAD 79
>gi|2463509|emb|CAA70735.1| (Y09541) pectate lyase [Zinnia elegans] Length = 401 Frame 3 hits (HSPs): _______ __________________________________________________ Database sequence: | | | | 401 0 150 300 Plus Strand HSPs: Score = 190 (66.9 bits), Expect = 2.3e-13, P = 2.3e-13 Identities = 34/51 (66%), Positives = 38/51 (74%), Frame = +3 Query: 309 PEEVVSMVEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 P +V+ V SI S RR LG+ SCGTGNPIDDCWRCDPNW NR+RLAD Sbjct: 26 PHAIVNEVHKSINAS--RRNLGYLSCGTGNPIDDCWRCDPNWANNRQRLAD 74
>gi|11072028|gb|AAG28907.1|AC008113_23 (AC008113) F12A21.12 [Arabidopsis thaliana] Length = 392 Frame 3 hits (HSPs): ______ __________________________________________________ Database sequence: | | | | 392 0 150 300 Plus Strand HSPs: Score = 172 (60.5 bits), Expect = 2.1e-11, P = 2.1e-11 Identities = 27/37 (72%), Positives = 31/37 (83%), Frame = +3 Query: 351 STERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 + RR LG SCGTGNPIDDCWRCDP W++NR+RLAD Sbjct: 29 NASRRNLGVLSCGTGNPIDDCWRCDPKWEKNRQRLAD 65
>gi|7939512|dbj|BAA95715.1| (AB024028) pectate lyase-like protein [Arabidopsis thaliana] Length = 409 Frame 3 hits (HSPs): ______ __________________________________________________ Database sequence: | | | | 409 0 150 300 Plus Strand HSPs: Score = 171 (60.2 bits), Expect = 3.0e-11, P = 3.0e-11 Identities = 29/46 (63%), Positives = 37/46 (80%), Frame = +3 Query: 324 SMVEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 S++ S+ N++ RR L + SC TGNPIDDCWRCDPNW+ NR+RLAD Sbjct: 38 SLICRSVFNAS-RRSLAYLSCRTGNPIDDCWRCDPNWETNRQRLAD 82
>gi|7449062|pir||T03932 pectate lyase (EC 4.2.2.2) Nt59 - common tobacco (fragment) >gi|2281330|gb|AAB69758.1| (U85646) putative pectate lyase Nt59 [Nicotiana tabacum] Length = 274 Frame 3 hits (HSPs): ________________ __________________________________________________ Database sequence: | | | | | | | 274 0 50 100 150 200 250 Plus Strand HSPs: Score = 156 (54.9 bits), Expect = 4.0e-10, P = 4.0e-10 Identities = 37/80 (46%), Positives = 44/80 (55%), Frame = +3 Query: 261 RAKEAEKLNEQAAVANPEEVV---------SMVEMSIQNSTERRKLGFFS----CGTGNP 401 RA+EA + Q NPE V SM E+++ N++ RR LG C NP Sbjct: 38 RAEEAREYARQIYEPNPENVTLAFNQKVRDSMKEVTVSNNSTRRGLGTKKYKGPCMVTNP 97 Query: 402 IDDCWRCDPNWQRNRKRLAD 461 ID CWRCDPNW NRKRLAD Sbjct: 98 IDRCWRCDPNWADNRKRLAD 117
>gi|11279704|pir||T46165 pectate lyase-like protein - Arabidopsis thaliana >gi|6630739|emb|CAB64222.1| (AL132958) pectate lyase-like protein [Arabidopsis thaliana] Length = 463 Frame 3 hits (HSPs): _______ __________________________________________________ Database sequence: | | | | | 463 0 150 300 450 Plus Strand HSPs: Score = 139 (48.9 bits), Expect = 1.1e-07, P = 1.1e-07 Identities = 28/59 (47%), Positives = 34/59 (57%), Frame = +3 Query: 306 NPEEVVSMVEMSIQNSTERRKL-------GFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 +PE V V+ + S RR+ G C TGNPIDDCW+CDPNW NR+ LAD Sbjct: 17 DPELVAYQVQWKVNASITRRQALDTTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLAD 75
>gi|7449065|pir||T06728 pectate lyase (EC 4.2.2.2) F28P10.100 - Arabidopsis thaliana >gi|4678301|emb|CAB41092.1| (AL049655) pectate lyase-like protein [Arabidopsis thaliana] Length = 542 Frame 3 hits (HSPs): ____ Frame 1 hits (HSPs): __ __________________________________________________ Database sequence: | | | | | 542 0 150 300 450 Plus Strand HSPs: Score = 123 (43.3 bits), Expect = 1.4e-07, Sum P(2) = 1.4e-07 Identities = 21/28 (75%), Positives = 23/28 (82%), Frame = +3 Query: 381 SCGTGNPIDDCWRC-DPNWQRNRKRLAD 461 SC TGNPIDDCWRC D +W NR+RLAD Sbjct: 74 SCRTGNPIDDCWRCSDADWSTNRQRLAD 101 Score = 38 (13.4 bits), Expect = 1.4e-07, Sum P(2) = 1.4e-07 Identities = 8/16 (50%), Positives = 11/16 (68%), Frame = +1 Query: 115 LLQQNGSSSCFWRCLF 162 +L QN S++ F CLF Sbjct: 1 MLLQNFSNTIFLLCLF 16
>gi|629849|pir||S43335 pectate lyase (EC 4.2.2.2) - maize >gi|405535|gb|AAA16476.1| (L20140) homology with pectate lyase [Zea mays] Length = 438 Frame 3 hits (HSPs): _________ Annotated Domains: ________________________________________________ __________________________________________________ Database sequence: | | | | 438 0 150 300 __________________ Annotated Domains: DOMO DM04067: 1..78 DOMO DM00584: PECTATELYASE 80..421 __________________ Plus Strand HSPs: Score = 129 (45.4 bits), Expect = 1.2e-06, P = 1.2e-06 Identities = 28/70 (40%), Positives = 37/70 (52%), Frame = +3 Query: 255 AERAKEAEKLNEQAAVANPEEVVSMVEMSIQNSTERRKLGFFS--CGTGNPIDDCWRCDP 428 AERA+ A N A V++P + + +T RR L + C NPID CWRC Sbjct: 42 AERAEVARSRNLAAYVSDPVAATNRFNADVLRATTRRALARYDGPCMATNPIDRCWRCRA 101 Query: 429 NWQRNRKRLA 458 +W +RKRLA Sbjct: 102 DWATDRKRLA 111
>gi|125889|sp|P15722|PE59_LYCES PROBABLE PECTATE LYASE P59 PRECURSOR >gi|280400|pir||S27098 pectate lyase (EC 4.2.2.2) LAT59 - tomato >gi|19271|emb|CAA33523.1| (X15499) P59 protein [Lycopersicon esculentum] Length = 449 Frame 3 hits (HSPs): ______ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | 449 0 150 300 __________________ Annotated Domains: DOMO DM04067: 1..86 DOMO DM00584: PECTATELYASE 88..435 Entrez active site: POTENTIAL. 325 Entrez glycosylation site: POTENTIAL. 56 Entrez glycosylation site: POTENTIAL. 80 Entrez glycosylation site: POTENTIAL. 81 PFAM pec_lyase: Pectate lyase 170..442 PRINTS AMBALLERGEN1: Pollen allergen AMB motif 122..139 PRINTS AMBALLERGEN2: Pollen allergen AMB motif 145..170 PRINTS AMBALLERGEN3: Pollen allergen AMB motif 181..197 PRINTS AMBALLERGEN4: Pollen allergen AMB motif 245..266 PRINTS AMBALLERGEN5: Pollen allergen AMB motif 325..344 PRINTS AMBALLERGEN6: Pollen allergen AMB motif 347..366 PRINTS AMBALLERGEN7: Pollen allergen AMB motif 389..413 PRINTS AMBALLERGEN8: Pollen allergen AMB motif 420..443 PRODOM PD013760: 10..90 PRODOM PD003200: PEL(2) 94..161 PRODOM PD117517: PE59_LYCES 163..189 PRODOM PD000974: PEL(9) PLYA(3) PLYD(3) 191..326 PRODOM PD003199: PEL(2) 328..403 PRODOM PD117525: PE59_LYCES 405..448 __________________ Plus Strand HSPs: Score = 124 (43.7 bits), Expect = 4.5e-06, P = 4.5e-06 Identities = 24/42 (57%), Positives = 27/42 (64%), Frame = +3 Query: 348 NSTERRKLGFFS----CGTGNPIDDCWRCDPNWQRNRKRLAD 461 N++ RR LG C NPID CWRCDPNW NRK+LAD Sbjct: 80 NNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLAD 121
>gi|9758606|dbj|BAB09239.1| (AB009050) pectate lyase [Arabidopsis thaliana] Length = 392 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 392 0 150 300 Plus Strand HSPs: Score = 120 (42.2 bits), Expect = 9.8e-06, P = 9.8e-06 Identities = 20/28 (71%), Positives = 22/28 (78%), Frame = +3 Query: 381 SCGT-GNPIDDCWRCDPNWQRNRKRLAD 461 SC GNPID+CWRCD NW+ NRK LAD Sbjct: 43 SCSANGNPIDECWRCDENWKDNRKNLAD 70
>gi|6094557|gb|AAF03499.1|AC010676_9 (AC010676) putative pectate lyase [Arabidopsis thaliana] >gi|6714461|gb|AAF26147.1|AC008261_4 (AC008261) putative pectate lyase [Arabidopsis thaliana] Length = 475 Frame 3 hits (HSPs): __________ __________________________________________________ Database sequence: | | | | | 475 0 150 300 450 Plus Strand HSPs: Score = 119 (41.9 bits), Expect = 1.7e-05, P = 1.7e-05 Identities = 31/79 (39%), Positives = 42/79 (53%), Frame = +3 Query: 258 ERAKEAEKLNEQAAVANPEE-VVSMVEMSIQNSTER--------RKLGFFS-----CGTG 395 + + + E E+ AVA+ EE V+ M+ S NST R + G +S C Sbjct: 66 DNSTDVEGTEEEKAVASEEEDVIEMIS-SPTNSTRRSLTGRGKGKGKGKWSKLTGPCTAS 124 Query: 396 NPIDDCWRCDPNWQRNRKRL 455 NPID CWRC P+W R RK+L Sbjct: 125 NPIDKCWRCQPDWARRRKKL 144
>gi|730290|sp|P40973|PEL_LILLO PECTATE LYASE PRECURSOR >gi|322883|pir||S29612 pectate lyase (EC 4.2.2.2) - trumpet lily >gi|19451|emb|CAA78976.1| (Z17328) pectate lyase [Lilium longiflorum] >gi|308902|gb|AAA33398.1| (L18911) pectate lyase [Lilium longiflorum] Length = 434 Frame 3 hits (HSPs): _________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | 434 0 150 300 __________________ Annotated Domains: DOMO DM04067: 1..73 DOMO DM00584: PECTATELYASE 75..417 Entrez active site: POTENTIAL. 312 Entrez glycosylation site: POTENTIAL. 68 Entrez glycosylation site: POTENTIAL. 97 PFAM pec_lyase: Pectate lyase 157..396 PRINTS AMBALLERGEN1: Pollen allergen AMB motif 108..125 PRINTS AMBALLERGEN2: Pollen allergen AMB motif 132..157 PRINTS AMBALLERGEN3: Pollen allergen AMB motif 168..184 PRINTS AMBALLERGEN4: Pollen allergen AMB motif 232..253 PRINTS AMBALLERGEN5: Pollen allergen AMB motif 312..331 PRINTS AMBALLERGEN6: Pollen allergen AMB motif 334..353 PRINTS AMBALLERGEN7: Pollen allergen AMB motif 376..400 PRINTS AMBALLERGEN8: Pollen allergen AMB motif 405..428 PRODOM PD013760: 6..74 PRODOM PD003200: PEL(2) 79..150 PRODOM PD000974: PEL(9) PLYA(3) PLYD(3) 152..313 PRODOM PD003199: PEL(2) 315..390 PRODOM PD117523: PEL_LILLO 392..433 __________________ Plus Strand HSPs: Score = 117 (41.2 bits), Expect = 4.9e-05, P = 4.9e-05 Identities = 31/73 (42%), Positives = 40/73 (54%), Frame = +3 Query: 258 ERAKEAEKLNEQAAVANPEEVVS----MVEMSIQ-NSTERR----KLGFFSCGTGNPIDD 410 +++K A+ ++A +PEEV + V S + NST R KLG C NPID Sbjct: 33 KKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEGNSTRRNLRTNKLG--QCLATNPIDR 90 Query: 411 CWRCDPNWQRNRKRL 455 CWRC NW NRK L Sbjct: 91 CWRCKKNWSANRKDL 105
>gi|112863|sp|P24396|9612_LYCES STYLE DEVELOPMENT-SPECIFIC PROTEIN 9612 PRECURSOR >gi|100227|pir||S12209 pectate lyase (EC 4.2.2.2) - tomato >gi|19162|emb|CAA38979.1| (X55193) 9612 [Lycopersicon esculentum] Length = 404 Frame 3 hits (HSPs): _____ Frame 2 hits (HSPs): ___ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | 404 0 150 300 __________________ Annotated Domains: DOMO DM00584: PECTATELYASE 43..387 Entrez glycosylation site: POTENTIAL. 37 Entrez glycosylation site: POTENTIAL. 191 PFAM pec_lyase: Pectate lyase 125..397 PRINTS AMBALLERGEN1: Pollen allergen AMB motif 76..93 PRINTS AMBALLERGEN2: Pollen allergen AMB motif 100..125 PRINTS AMBALLERGEN3: Pollen allergen AMB motif 136..152 PRINTS AMBALLERGEN4: Pollen allergen AMB motif 200..221 PRINTS AMBALLERGEN5: Pollen allergen AMB motif 280..299 PRINTS AMBALLERGEN6: Pollen allergen AMB motif 302..321 PRINTS AMBALLERGEN7: Pollen allergen AMB motif 344..368 PRINTS AMBALLERGEN8: Pollen allergen AMB motif 375..398 PRODOM PD117527: 9612_LYCES 1..47 PRODOM PD003200: PEL(2) 49..117 PRODOM PD191029: 9612(1) O24554(1) 119..140 PRODOM PD000974: PEL(9) PLYA(3) PLYD(3) 142..281 PRODOM PD003199: PEL(2) 283..358 PRODOM PD023601: 9612(1) O23017(1) O24554(1) 364..403 __________________ Plus Strand HSPs: Score = 102 (35.9 bits), Expect = 0.00062, Sum P(2) = 0.00062 Identities = 20/38 (52%), Positives = 26/38 (68%), Frame = +3 Query: 294 AAVANPEEVVSMVEMSIQNSTERRKLGFFSCGTGNPID 407 +++ +PE VV V SI S RR LG+ SCG+GNPID Sbjct: 20 SSIQDPELVVQDVHRSINASLTRRNLGYLSCGSGNPID 57 Score = 46 (16.2 bits), Expect = 0.00062, Sum P(2) = 0.00062 Identities = 11/21 (52%), Positives = 14/21 (66%), Frame = +2 Query: 410 LLALRPQLATQSEASCRLAIG 472 LLA++PQL +S A AIG Sbjct: 59 LLAMQPQLGKKSPAFSYCAIG 79
>gi|2281334|gb|AAB69759.1| (U83619) putative pectate lyase [Arabidopsis thaliana] Length = 459 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | | 459 0 150 300 450 Plus Strand HSPs: Score = 112 (39.4 bits), Expect = 0.00083, P = 0.00083 Identities = 18/26 (69%), Positives = 20/26 (76%), Frame = +3 Query: 384 CGTGNPIDDCWRCDPNWQRNRKRLAD 461 C NPID+CWRCD NW NRK+LAD Sbjct: 101 CLAYNPIDNCWRCDRNWANNRKKLAD 126
>gi|7262684|gb|AAF43942.1|AC012188_19 (AC012188) Strong similarity to Pectate Lyase Precursor from Lilium longiflorum gi|730290 and contains a Pectate lyase PF|00544 domain. EST gb|AW004514 comes from this gene. [Arabidopsis thaliana] Length = 459 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | | 459 0 150 300 450 Plus Strand HSPs: Score = 112 (39.4 bits), Expect = 0.00083, P = 0.00083 Identities = 18/26 (69%), Positives = 20/26 (76%), Frame = +3 Query: 384 CGTGNPIDDCWRCDPNWQRNRKRLAD 461 C NPID+CWRCD NW NRK+LAD Sbjct: 101 CLAYNPIDNCWRCDRNWANNRKKLAD 126
>gi|730291|sp|P40972|PEL_TOBAC PECTATE LYASE PRECURSOR >gi|280405|pir||S26211 pectate lyase (EC 4.2.2.2) - common tobacco >gi|19908|emb|CAA47630.1| (X67158) pectate lyase [Nicotiana tabacum] >gi|19982|emb|CAA43414.1| (X61102) pectate lyase [Nicotiana tabacum] Length = 397 Frame 3 hits (HSPs): ____ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | 397 0 150 300 __________________ Annotated Domains: Entrez active site: POTENTIAL. 272 Entrez glycosylation site: POTENTIAL. 134 Entrez glycosylation site: POTENTIAL. 227 PFAM pec_lyase: Pectate lyase 117..351 PRINTS AMBALLERGEN1: Pollen allergen AMB motif 68..85 PRINTS AMBALLERGEN2: Pollen allergen AMB motif 92..117 PRINTS AMBALLERGEN3: Pollen allergen AMB motif 128..144 PRINTS AMBALLERGEN4: Pollen allergen AMB motif 192..213 PRINTS AMBALLERGEN5: Pollen allergen AMB motif 272..291 PRINTS AMBALLERGEN6: Pollen allergen AMB motif 294..313 PRINTS AMBALLERGEN7: Pollen allergen AMB motif 338..362 PRINTS AMBALLERGEN8: Pollen allergen AMB motif 368..391 PRODOM PD117520: PEL_TOBAC 1..38 PRODOM PD003200: PEL(2) 40..108 PRODOM PD000974: PEL(9) PLYA(3) PLYD(3) 129..273 PRODOM PD003199: PEL(2) 275..352 PRODOM PD038458: PE56(1) PEL(1) 354..396 __________________ Plus Strand HSPs: Score = 108 (38.0 bits), Expect = 0.0028, P = 0.0028 Identities = 16/26 (61%), Positives = 19/26 (73%), Frame = +3 Query: 384 CGTGNPIDDCWRCDPNWQRNRKRLAD 461 C N ID CWRCDPNW NR+++AD Sbjct: 42 CRAENAIDKCWRCDPNWAENRQKMAD 67
>gi|9087152|sp|P81294|MPA1_JUNAS MAJOR POLLEN ALLERGEN JUN A 1 PRECURSOR >gi|4138877|gb|AAD03608.1| (AF106662) pollen major allergen 1-1 [Juniperus ashei] >gi|4138879|gb|AAD03609.1| (AF106663) pollen major allergen 1-2 [Juniperus ashei] Length = 367 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 367 0 150 300 Plus Strand HSPs: Score = 104 (36.6 bits), Expect = 0.0096, P = 0.0095 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCYSDNPIDSCWRGDSNWDQNRMKLAD 44
>gi|8101711|gb|AAF72625.1|AF257491_1 (AF257491) Cup s 1 pollen allergen precursor [Cupressus sempervirens] Length = 367 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 367 0 150 300 Plus Strand HSPs: Score = 104 (36.6 bits), Expect = 0.0096, P = 0.0095 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCYSDNPIDSCWRGDSNWDQNRMKLAD 44
>gi|8101713|gb|AAF72626.1|AF257492_1 (AF257492) Cup s 1 pollen allergen precursor [Cupressus sempervirens] Length = 367 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 367 0 150 300 Plus Strand HSPs: Score = 104 (36.6 bits), Expect = 0.0096, P = 0.0095 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCYSDNPIDSCWRGDSNWDQNRMKLAD 44
>gi|8101715|gb|AAF72627.1|AF257493_1 (AF257493) Cup s 1 pollen allergen precursor [Cupressus sempervirens] Length = 367 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 367 0 150 300 Plus Strand HSPs: Score = 104 (36.6 bits), Expect = 0.0096, P = 0.0095 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCYSDNPIDSCWRGDSNWDQNRMKLAD 44
>gi|8101717|gb|AAF72628.1|AF257494_1 (AF257494) Cup s 1 pollen allergen precursor [Cupressus sempervirens] Length = 367 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 367 0 150 300 Plus Strand HSPs: Score = 104 (36.6 bits), Expect = 0.0096, P = 0.0095 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCYSDNPIDSCWRGDSNWDQNRMKLAD 44
>gi|8101719|gb|AAF72629.1|AF257495_1 (AF257495) Cup s 1 pollen allergen precursor [Cupressus sempervirens] Length = 367 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 367 0 150 300 Plus Strand HSPs: Score = 104 (36.6 bits), Expect = 0.0096, P = 0.0095 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCYSDNPIDSCWRGDSNWDQNRMKLAD 44
>gi|9087163|sp|Q96385|MPA1_CHAOB MAJOR POLLEN ALLERGEN CHA O 1 PRECURSOR >gi|1514943|dbj|BAA08246.1| (D45404) Chao1 [Chamaecyparis obtusa] Length = 375 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 375 0 150 300 Plus Strand HSPs: Score = 104 (36.6 bits), Expect = 0.0099, P = 0.0098 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCFSDNPIDSCWRGDANWDQNRMKLAD 44
>gi|1173367|sp|P18632|SBP_CRYJA SUGI BASIC PROTEIN PRECURSOR (SBP) (MAJOR ALLERGEN CRY J 1) (CRY J I) >gi|541803|pir||JC2123 major allergen Cry j I precursor (clone pCCI-2-2) - Japanese cedar >gi|493632|dbj|BAA05542.1| (D26544) Cry j IA precursor [Cryptomeria japonica] Length = 374 Frame 3 hits (HSPs): ____ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | 374 0 150 300 __________________ Annotated Domains: DOMO DM00584: PECTATELYASE 12..357 Entrez glycosylation site: POTENTIAL. 158 Entrez glycosylation site: POTENTIAL. 191 Entrez glycosylation site: POTENTIAL. 293 PFAM pec_lyase: Pectate lyase 96..367 PRINTS AMBALLERGEN1: Pollen allergen AMB motif 45..62 PRINTS AMBALLERGEN2: Pollen allergen AMB motif 68..93 PRINTS AMBALLERGEN3: Pollen allergen AMB motif 104..120 PRINTS AMBALLERGEN4: Pollen allergen AMB motif 170..191 PRINTS AMBALLERGEN5: Pollen allergen AMB motif 250..269 PRINTS AMBALLERGEN6: Pollen allergen AMB motif 272..291 PRINTS AMBALLERGEN7: Pollen allergen AMB motif 315..339 PRINTS AMBALLERGEN8: Pollen allergen AMB motif 345..368 PRODOM PD003200: PEL(2) 1..86 PRODOM PD000974: PEL(9) PLYA(3) PLYD(3) 88..251 PRODOM PD003199: PEL(2) 253..329 PRODOM PD038459: Q96385(1) Q9ZNU7(1) SBP(1) 331..366 __________________ Plus Strand HSPs: Score = 103 (36.3 bits), Expect = 0.013, P = 0.013 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCFSDNPIDSCWRGDSNWAQNRMKLAD 44
>gi|541802|pir||JC2124 major allergen Cry j I precursor (clone pCCI-15) - Japanese cedar >gi|493634|dbj|BAA05543.1| (D26545) Cry j IB precursor [Cryptomeria japonica] Length = 374 Frame 3 hits (HSPs): ____ Annotated Domains: ___ _ _ _ _ __________________________________________________ Database sequence: | | | | 374 0 150 300 __________________ Annotated Domains: Entrez domain: signal sequence 1..21 Entrez binding site: carbohydrate (Asn) (covale 158 Entrez binding site: carbohydrate (Asn) (covale 191 Entrez binding site: carbohydrate (Asn) (covale 293 Entrez binding site: carbohydrate (Asn) (covale 354 __________________ Plus Strand HSPs: Score = 103 (36.3 bits), Expect = 0.013, P = 0.013 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCFSDNPIDSCWRGDSNWAQNRMKLAD 44
>gi|516728|dbj|BAA07020.1| (D34639) Cry j I precursor [Cryptomeria japonica] Length = 374 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 374 0 150 300 Plus Strand HSPs: Score = 103 (36.3 bits), Expect = 0.013, P = 0.013 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCFSDNPIDSCWRGDSNWAQNRMKLAD 44
>gi|8843917|gb|AAF80164.1| (AF151427) pollen major allergen 1-2 [Juniperus virginiana] Length = 367 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 367 0 150 300 Plus Strand HSPs: Score = 102 (35.9 bits), Expect = 0.017, P = 0.017 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCCSDNPIDSCWRGDSNWGQNRMKLAD 44
>gi|8843921|gb|AAF80166.1| (AF151429) pollen major allergen 1-1 [Juniperus virginiana] Length = 367 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 367 0 150 300 Plus Strand HSPs: Score = 102 (35.9 bits), Expect = 0.017, P = 0.017 Identities = 17/27 (62%), Positives = 20/27 (74%), Frame = +3 Query: 381 SCGTGNPIDDCWRCDPNWQRNRKRLAD 461 SC + NPID CWR D NW +NR +LAD Sbjct: 18 SCCSDNPIDSCWRGDSNWGQNRMKLAD 44
>gi|11358602|pir||T51456 pectate lyase-like protein - Arabidopsis thaliana >gi|9755678|emb|CAC01830.1| (AL391146) pectate lyase-like protein [Arabidopsis thaliana] Length = 472 Frame 3 hits (HSPs): ________ __________________________________________________ Database sequence: | | | | | 472 0 150 300 450 Plus Strand HSPs: Score = 96 (33.8 bits), Expect = 0.15, P = 0.14 Identities = 27/68 (39%), Positives = 34/68 (50%), Frame = +3 Query: 288 EQAAVANPEEVVS-MVEM--SIQNSTER----RKLGFFS-----CGTGNPIDDCWRCDPN 431 E+ A PEE +EM S NST R + G +S C NPID CWRC + Sbjct: 74 EEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSD 133 Query: 432 WQRNRKRL 455 W + RK+L Sbjct: 134 WAKRRKKL 141
>gi|9087167|sp|Q9SCG9|MPA1_CUPAR MAJOR POLLEN ALLERGEN CUP A 1 >gi|6562326|emb|CAB62551.1| (AJ243570) cup a 1 protein [Cupressus arizonica] Length = 346 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | | 346 0 150 300 Plus Strand HSPs: Score = 94 (33.1 bits), Expect = 0.17, P = 0.16 Identities = 15/22 (68%), Positives = 17/22 (77%), Frame = +3 Query: 396 NPIDDCWRCDPNWQRNRKRLAD 461 NPID CWR D NW +NR +LAD Sbjct: 2 NPIDSCWRGDSNWDQNRMKLAD 23
>gi|113478|sp|P28744|MP14_AMBAR POLLEN ALLERGEN AMB A 1.4 PRECURSOR (ANTIGEN E) (ANTIGEN AMB A I) >gi|539050|pir||D53240 allergen Amb a I.4 precursor - common ragweed >gi|166445|gb|AAA32670.1| (M80562) major allergen [Ambrosia artemisiifolia] Length = 392 Frame 3 hits (HSPs): ______ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | 392 0 150 300 __________________ Annotated Domains: Entrez blocked site 1 PFAM pec_lyase: Pectate lyase 120..349 PRINTS AMBALLERGEN1: Pollen allergen AMB motif 71..88 PRINTS AMBALLERGEN2: Pollen allergen AMB motif 95..120 PRINTS AMBALLERGEN3: Pollen allergen AMB motif 131..147 PRINTS AMBALLERGEN4: Pollen allergen AMB motif 194..215 PRINTS AMBALLERGEN5: Pollen allergen AMB motif 270..289 PRINTS AMBALLERGEN6: Pollen allergen AMB motif 292..311 PRINTS AMBALLERGEN7: Pollen allergen AMB motif 333..357 PRINTS AMBALLERGEN8: Pollen allergen AMB motif 363..386 PRODOM PD013758: 1..40 PRODOM PD003200: PEL(2) 44..113 PRODOM PD000974: PEL(9) PLYA(3) PLYD(3) 121..271 PRODOM PD003199: PEL(2) 275..347 PRODOM PD013759: 349..391 __________________ Plus Strand HSPs: Score = 94 (33.1 bits), Expect = 0.21, P = 0.19 Identities = 19/44 (43%), Positives = 26/44 (59%), Frame = +3 Query: 330 VEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 ++ + ++ E R L +CGT N ID CWR +W NRK LAD Sbjct: 29 LQQILPSANETRSLT--TCGTYNIIDGCWRGKADWAENRKALAD 70
>gi|539051|pir||E53240 allergen Amb a II precursor - common ragweed Length = 397 Frame 3 hits (HSPs): _____ __________________________________________________ Database sequence: | | | | 397 0 150 300 Plus Strand HSPs: Score = 93 (32.7 bits), Expect = 0.28, P = 0.25 Identities = 18/38 (47%), Positives = 20/38 (52%), Frame = +3 Query: 348 NSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 N T R G C N ID CWRC P+W NR+ L D Sbjct: 36 NDTRRSLQG---CEAHNIIDKCWRCKPDWAENRQALGD 70
>gi|113476|sp|P27760|MP12_AMBAR POLLEN ALLERGEN AMB A 1.2 PRECURSOR (ANTIGEN E) (ANTIGEN AMB A I) >gi|100497|pir||B39099 allergen Amb a I.2 - common ragweed >gi|166437|gb|AAA32666.1| (M62981) Amb a I.2 [Ambrosia artemisiifolia] >gi|166439|gb|AAA32667.1| (M80559) Amb a I.2 precursor protein [Ambrosia artemisiifolia] Length = 398 Frame 3 hits (HSPs): ______ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | 398 0 150 300 __________________ Annotated Domains: DOMO DM00584: PECTATELYASE 39..381 Entrez blocked site 1 PFAM pec_lyase: Pectate lyase 121..359 PRINTS AMBALLERGEN1: Pollen allergen AMB motif 72..89 PRINTS AMBALLERGEN2: Pollen allergen AMB motif 96..121 PRINTS AMBALLERGEN3: Pollen allergen AMB motif 132..148 PRINTS AMBALLERGEN4: Pollen allergen AMB motif 195..216 PRINTS AMBALLERGEN5: Pollen allergen AMB motif 275..294 PRINTS AMBALLERGEN6: Pollen allergen AMB motif 297..316 PRINTS AMBALLERGEN7: Pollen allergen AMB motif 339..363 PRINTS AMBALLERGEN8: Pollen allergen AMB motif 369..392 PRODOM PD013758: 1..40 PRODOM PD003200: PEL(2) 45..114 PRODOM PD000974: PEL(9) PLYA(3) PLYD(3) 133..276 PRODOM PD003199: PEL(2) 278..353 PRODOM PD013759: 355..397 __________________ Plus Strand HSPs: Score = 93 (32.7 bits), Expect = 0.29, P = 0.25 Identities = 18/44 (40%), Positives = 25/44 (56%), Frame = +3 Query: 330 VEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 VE + ++ E R+ +C N ID CWRC +W NR+ LAD Sbjct: 29 VEEFLPSANETRR-SLKACEAHNIIDKCWRCKADWANNRQALAD 71
>gi|539048|pir||B53240 allergen Amb a I.2 precursor - common ragweed Length = 398 Frame 3 hits (HSPs): ______ Annotated Domains: ____________________________________________ __________________________________________________ Database sequence: | | | | 398 0 150 300 __________________ Annotated Domains: DOMO DM00584: PECTATELYASE 39..381 __________________ Plus Strand HSPs: Score = 93 (32.7 bits), Expect = 0.29, P = 0.25 Identities = 18/44 (40%), Positives = 25/44 (56%), Frame = +3 Query: 330 VEMSIQNSTERRKLGFFSCGTGNPIDDCWRCDPNWQRNRKRLAD 461 VE + ++ E R+ +C N ID CWRC +W NR+ LAD Sbjct: 29 VEEFLPSANETRR-SLKACEAHNIIDKCWRCKADWANNRQALAD 71
>gi|1352734|sp|P15721|PE56_LYCES PROBABLE PECTATE LYASE P56 PRECURSOR >gi|7449061|pir||T07058 pectate lyase (EC 4.2.2.2) LAT56 - tomato >gi|551656|emb|CAA33524.1| (X15500) P56 protein [Lycopersicon esculentum] Length = 398 Frame 3 hits (HSPs): ____ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | 398 0 150 300 __________________ Annotated Domains: DOMO DM00584: PECTATELYASE 36..381 Entrez active site: POTENTIAL. 273 Entrez glycosylation site: POTENTIAL. 135 Entrez glycosylation site: POTENTIAL. 228 PFAM pec_lyase: Pectate lyase 118..314 PRINTS AMBALLERGEN1: Pollen allergen AMB motif 69..86 PRINTS AMBALLERGEN2: Pollen allergen AMB motif 93..118 PRINTS AMBALLERGEN3: Pollen allergen AMB motif 129..145 PRINTS AMBALLERGEN4: Pollen allergen AMB motif 193..214 PRINTS AMBALLERGEN5: Pollen allergen AMB motif 273..292 PRINTS AMBALLERGEN6: Pollen allergen AMB motif 295..314 PRINTS AMBALLERGEN7: Pollen allergen AMB motif 339..363 PRINTS AMBALLERGEN8: Pollen allergen AMB motif 369..392 PRODOM PD117521: PE56_LYCES 1..39 PRODOM PD003200: PEL(2) 41..109 PRODOM PD000974: PEL(9) PLYA(3) PLYD(3) 119..274 PRODOM PD003199: PEL(2) 276..353 PRODOM PD038458: PE56(1) PEL(1) 355..397 __________________ Plus Strand HSPs: Score = 92 (32.4 bits), Expect = 0.39, P = 0.32 Identities = 14/26 (53%), Positives = 18/26 (69%), Frame = +3 Query: 384 CGTGNPIDDCWRCDPNWQRNRKRLAD 461 C N ID CWRCDP W +R+++AD Sbjct: 43 CMAVNSIDKCWRCDPFWAEDRQKMAD 68
>gi|7449064|pir||T00856 pectate lyase (EC 4.2.2.2) T20F6.14 - Arabidopsis thaliana >gi|2947069|gb|AAC05350.1| (AC002521) putative pectate lyase [Arabidopsis thaliana] Length = 455 Frame 3 hits (HSPs): ____ __________________________________________________ Database sequence: | | | || 455 0 150 300 450 Plus Strand HSPs: Score = 92 (32.4 bits), Expect = 0.47, P = 0.37 Identities = 15/22 (68%), Positives = 17/22 (77%), Frame = +3 Query: 396 NPIDDCWRCDPNWQRNRKRLAD 461 N ID CWR D NW +NRK+LAD Sbjct: 101 NAIDKCWRGDKNWDKNRKKLAD 122
>gi|280372|pir||A44773 pollen allergen I - Japanese cedar (fragment) Length = 20 Frame 3 hits (HSPs): ________________________________________________ __________________________________________________ Database sequence: | | 20 0 Plus Strand HSPs: Score = 68 (23.9 bits), Expect = 0.94, P = 0.61 Identities = 11/19 (57%), Positives = 13/19 (68%), Frame = +3 Query: 396 NPIDDCWRCDPNWQRNRKR 452 NPID WR D NW +NR + Sbjct: 2 NPIDSXWRGDSNWAQNRMK 20 WARNING: HSPs involving 16 database sequences were not reported due to the limiting value of parameter B = 50. Parameters: filter=none matrix=BLOSUM62 V=50 B=50 E=10 gi H=1 sort_by_pvalue echofilter ctxfactor=5.91 Query ----- As Used ----- ----- Computed ---- Frame MatID Matrix name Lambda K H Lambda K H Std. 0 BLOSUM62 0.318 0.135 0.401 +3 0 BLOSUM62 0.316 0.126 0.362 same same same Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a +2 0 BLOSUM62 0.318 0.135 0.401 0.346 0.153 0.517 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a +1 0 BLOSUM62 0.318 0.135 0.401 0.338 0.143 0.497 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a -1 0 BLOSUM62 0.318 0.135 0.401 0.342 0.147 0.527 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a -2 0 BLOSUM62 0.318 0.135 0.401 0.323 0.132 0.369 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a -3 0 BLOSUM62 0.318 0.135 0.401 0.340 0.147 0.505 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a Query Frame MatID Length Eff.Length E S W T X E2 S2 +3 0 162 161 10. 75 3 12 22 0.095 34 30 0.10 37 +2 0 162 161 10. 75 3 12 22 0.10 34 30 0.10 37 +1 0 162 161 10. 75 3 12 22 0.10 34 30 0.10 37 -1 0 162 162 10. 75 3 12 22 0.10 34 30 0.10 37 -2 0 162 161 10. 75 3 12 22 0.10 34 30 0.10 37 -3 0 162 161 10. 75 3 12 22 0.10 34 30 0.10 37 Statistics: Database: /usr/local/dot5/sl_home/beauty/seqdb/blast/nr Title: nr Release date: unknown Posted date: 4:06 PM CST Feb 28, 2001 Format: BLAST # of letters in database: 197,782,623 # of sequences in database: 625,274 # of database sequences satisfying E: 66 No. of states in DFA: 590 (58 KB) Total size of DFA: 198 KB (256 KB) Time to generate neighborhood: 0.01u 0.00s 0.01t Elapsed: 00:00:00 No. of threads or processors used: 6 Search cpu time: 174.17u 0.99s 175.16t Elapsed: 00:00:30 Total cpu time: 174.21u 1.03s 175.24t Elapsed: 00:00:30 Start: Tue Feb 5 00:57:44 2002 End: Tue Feb 5 00:58:14 2002 WARNINGS ISSUED: 2
Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000