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00.056.0.81.020. Wineberry latent virus

Cite this publication as: ICTVdB Management (2006). 00.056.0.81.020. Wineberry latent virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Scotland in imported material; the United Kingdom.

Host of Isolate and Habitat Details
Source of isolate: Rubus phoenicolasius.

Natural host and symptoms
Rubus phoenicolasius — symptomless. However, it has been shown to induce calico disease-like symptoms in blackberry.

Reference to Isolation Report
Jones (1977, Jones et al. (1990).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.056.0.81.020. Virus accession number: 56081020. Obsolete virus code: 56.0.1.T.020; superceded accession number: 5601t020.

Name, Synonyms and Lineage

Synonym(s): loganberry calico virus. ICTV approved acronym: WLV. Virus is a tentative member of the genus 00.056.0.01. Potexvirus; family 00.056. Flexiviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped. Capsid/nucleocapsid is elongated with helical symmetry. The capsid is filamentous, flexuous with a length of 620 nm and a width of 12 nm. Axial canal is indistinct. Basic helix is obscure.

Electron microscopic preparation and references: Virus preparation contains few virions. Reference for electron microscopic methods: Jones et al. (1990).

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.29 and 1.3 g cm-3. The density of virions is 1.25 and 1.26 g/cm3 in Cs2SO4. There are sometimes 2 sedimenting component(s) found in purified preparations. The sedimentation coefficient is 115-125 S20w. A260/A280 ratio is 1.26. The thermal inactivation point (TIP) is at less than 70°C. The longevity in vitro (LIV) is less than 8 days (at 18°C). Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 3-4. The infectivity is not changed by treatment with ether.

Nucleic Acid

The Mr of the genome constitutes 5% of the virion by weight. The genome is monopartite, only one particle size is recovered of linear, positive-sense, single-stranded RNA. Minor species of non-genomic nucleic acid are not found in virions. The complete genome is 8500 nucleotides long. Reference to nucleotide sequence isolation method by Jones: proteinase K + phenol.

Proteins

Proteins constitute about 95% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s).

Structural Proteins: Reference to method of preparation: Jones et al. (1990).

Lipids

Lipids are not reported.

Transcription: The virus codes for 5 ORF(s).

The genome expression is based on RNA production which can be analyzed by the dsRNA patterns found in the infected tissues. Usually there are 1 virus specified dsRNA species found in infected cells. Size of largest virus specified dsRNA 17 kbp.

Coding Strategy of Segment 1: Sequence has a gene block. Sequence has triple gene block sequence (TGB). Encodes proteins involved in cell to cell movement.

Antigenicity

The virus does not show serological relationships to 9 carlaviruses.

Diagnostics and Reference Collections

The best tests for diagnosis are ISEM and mechanical inoculation to Chenopodium quinoa.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

General Symptoms in Plants Symptoms chlorotic or yellow line pattern.

Severity and Occurrence of Disease

Host: Signs and symptoms vary seasonally.

Transmission and Vector Relationships

Virus is transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between hosts; not transmitted by seeds; not transmitted by pollen.

Vector Transmission: Virus is not transmitted by Macrosiphum euphorbiae.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Amaranthaceae, Chenopodiaceae, Rosaceae, Solanaceae, Tetragoniaceae. The following species were susceptible to experimental virus infection: Beta macrocarpa, Chenopodium album, Chenopodium amaranticolor, Chenopodium ambrosioides, Chenopodium foetidum, Chenopodium murale, Chenopodium quinoa, Gomphrena globosa, Lycopersicon esculentum, Rubus phoenicolasius, Spinacia oleracea, Tetragonia tetragonioides.

Host:
Experimentally infected hosts mainly show symptoms of necrotic or red local lesions.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Leguminosae-Papilionoideae, Solanaceae, or Umbelliferae. Species inoculated with virus that do not show signs of susceptibility: Apium graveolens, Datura stramonium, Nicotiana clevelandii, Nicotiana debneyi, Nicotiana glutinosa, Nicotiana rustica, Nicotiana tabacum, Petunia x hybrida, Phaseolus vulgaris, Pisum sativum, Vicia faba.

Diagnostic Hosts

Diagnostic host species and symptoms:

Gomphrena globosa, Chenopodium album, C. amaranticolor, C. ambrosioides, C. murale, C. quinoa — necrotic local lesions.

Diagnostic host: insusceptible host species Nicotiana clevelandii, N. debneyi, N. glutinosa, N. tabacum, Phaseolus vulgaris.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Chenopodium amaranticolor, C. quinoa, Gomphrena globosa.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Chenopodium amaranticolor (L), C. quinoa (L).

References to host data: Jones (1977).

Histopathology: Virus can be best detected in leaves and all parts of the host plant.

Geographical Distribution

The virus occurs in the United States of America. The virus is found, but with no evidence of proliferation, in the U.K.

References

Jones, AT (1977). Ann. appl. Biol. 86: 199.

Jones, AT (1985). AAB Descr. Pl. Viruses No. 304.

Jones, AT, Mitchell, M.J., McGavin, W.J. and Roberts, I.M. (1990). Ann. appl. Biol. 117: 571.
The following generic references are cited in the most recent ICTV Report.
A description of this taxon in VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 900 by A.T. Jones, 1991. A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 304.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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