RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pleuronectes vetulus
FDA Seafood List Common Name: English Sole
FDA Seafood List Market Name: Sole
Unique IEF Gel Code: 19950831gel06
Unique Web Code: esh01Srr
Tissue Aliquot Code: pleuvetu I94-002 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	68	8.4	0.020821	7	0.254657	15.6	9.7	4.55	68	7.2	0.0198325
2	5.2	169	20.8	0.0114802	8	0.151448	9.3	5.8	5.2	168.5	7.7	0.0111295
3	5.85	267	32.9	0.0194061	6	0.198608	12.1	7.5	5.85	267.6	5.9	0.0189438
4	6.55	386	47.5	0.0148143	10	0.255601	15.6	9.7	6.55	382.5	10.5	0.0137726
5	8.15	597	73.5	0.0178503	8	0.238809	14.6	9.1	8.15	595.7	8.1	0.0166281
6	8.45	664	81.8	0.0154571	7	0.185718	11.4	7.1	8.45	662.9	7.2	0.0144744
7	8.65	715	88.1	0.0126756	8	0.146656	9	5.6	8.65	714.7	8.4	0.00984105
8	9.3	786	96.8	0.0136316	10	0.204417	12.5	7.8	9.3	785.4	9.8	0.0117745

PeakCounter: 8

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.246	16	2	0.0218834	6	0.234971	1.1	0.8	4.246	17.7	6	0.0220323
2	4.322	28	3.5	0.0988408	3	0.331993	1.6	1.2	4.322	27.6	3	0.0634135
3	4.423	44	5.4	0.239189	16	6.46645	31.3	23.2	4.423	49.3	16.2	0.225033
4	5.108	152	18.8	0.0205701	4	0.133187	0.6	0.5	5.108	152.2	3.6	0.0211325
5	5.248	174	21.5	0.035575	4	0.231482	1.1	0.8	5.248	173.9	3.8	0.0345627
6	5.435	203	25.1	0.288195	5	2.26852	11	8.1	5.435	202.7	4.5	0.284323
7	5.512	215	26.5	0.116855	5	0.959518	4.7	3.4	5.512	214.7	4.7	0.114743
8	5.589	227	28	0.00748204	2	0.0116361	0.1	0	5.589	227.5	1.5	0.00434678
9	5.686	242	29.9	0.257304	5	2.35431	11.4	8.5	5.686	241.8	5.1	0.257923
10	5.763	254	31.4	0.078824	5	0.612541	3	2.2	5.763	253.4	4.9	0.0701084
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.834	265	32.7	0.069101	9	0.976223	4.7	3.5	5.834	265.7	8.5	0.0647423
12	5.896	275	34	0.182992	5	1.53481	7.4	5.5	5.896	275.1	5.2	0.165252
13	6.001	292	36	0.0111411	2	0.0407708	0.2	0.1	6.001	292.1	2.2	0.0104402
14	6.291	339	41.9	0.096958	9	1.52965	7.4	5.5	6.291	338	9.3	0.0930944
15	6.408	358	44.2	0.0530034	3	0.234682	1.1	0.8	6.408	358.1	3	0.043953
16	7.136	459	56.7	0.112889	13	2.70652	13.1	9.7	7.136	455.7	12.8	0.118951

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	63	7.8	0.0279327	7	0.328349	18.2	11.2	4.55	64	6.8	0.027103
2	5.2	167	20.6	0.0155743	9	0.216049	12	7.4	5.2	166.9	8.8	0.01384
3	5.85	271	33.4	0.0208405	8	0.277247	15.4	9.4	5.85	270.6	8	0.0195875
4	6.55	379	46.7	0.0256576	14	0.576976	32	19.6	6.55	375.5	13.5	0.0240814
5	8.15	594	73.2	0.0193852	5	0.149353	8.3	5.1	8.15	595.1	5.3	0.0157991
6	8.45	659	81.3	0.0109916	7	0.118757	6.6	4	8.45	657.6	7.1	0.00949283
7	8.65	705	86.9	0.00814047	7	0.0801835	4.4	2.7	8.65	705	7.1	0.00633783
8	9.3	777	95.8	0.00485089	10	0.0584963	3.2	2	9.3	772.6	9.5	0.00346428

PeakCounter: 8

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.311	29	3.6	0.00939345	8	0.172542	1.2	0.9	4.311	34.4	8.2	0.011852
2	4.416	46	5.7	0.0407173	5	0.262166	1.9	1.4	4.416	45.2	4.9	0.030354
3	4.521	63	7.8	0.150174	13	3.41322	24.4	18.7	4.521	67.1	12.8	0.150844
4	5.126	161	19.8	0.0123904	6	0.115158	0.8	0.6	5.126	160.9	6.2	0.0104317
5	5.2	173	21.3	0.0106483	4	0.0745551	0.5	0.4	5.2	172.3	4	0.0105234
6	5.258	182	22.4	0.0232726	4	0.141597	1	0.8	5.258	182.3	3.6	0.0224391
7	5.457	213	26.2	0.190379	5	1.70767	12.2	9.3	5.457	213.6	5.2	0.185269
8	5.553	228	28.1	0.0850343	6	0.804948	5.8	4.4	5.553	227.2	5.6	0.0807507
9	5.732	256	31.5	0.232392	4	1.57435	11.3	8.6	5.732	256.2	3.8	0.233499
10	5.816	269	33.1	0.0492746	3	0.290195	2.1	1.6	5.816	269.5	3.4	0.0481374
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.904	282	34.7	0.0591241	5	0.553057	4	3	5.904	282.2	5.5	0.0571496
12	5.968	291	35.8	0.112186	4	0.869017	6.2	4.8	5.968	291.6	4.5	0.10898
13	6.096	309	38.1	0.00279193	1	0	0	0	6.096	307	1	0
14	6.337	343	42.2	0.107895	9	1.65696	11.9	9.1	6.337	342.2	9.2	0.101448
15	6.444	358	44.1	0.0886127	4	0.503758	3.6	2.8	6.444	358.7	3.8	0.0742932
16	6.55	373	45.9	0.0364935	11	0.70391	5	3.9	6.55	372.5	11	0.0360214
17	7.199	464	57.1	0.0478108	13	1.12457	8.1	6.2	7.199	462.6	12.9	0.0493295

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.257	27	3.3	0.00865509	6	0.0955815	0.6	0.4	4.257	25.9	6.4	0.00838795
2	4.335	39	4.8	0.0490526	2	0.189384	1.2	0.9	4.335	38.9	2.2	0.0491876
3	4.451	57	7	0.17368	8	2.28287	14.7	10.4	4.451	61.3	8.2	0.156662
4	5.071	153	18.8	0.0168898	3	0.0976919	0.6	0.4	5.071	153.1	3.3	0.0166333
5	5.206	174	21.4	0.0291313	3	0.172604	1.1	0.8	5.206	174.1	3.4	0.0290061
6	5.38	202	24.8	0.307168	4	2.35864	15.2	10.8	5.38	202.9	4.5	0.298365
7	5.473	217	26.7	0.1394	6	1.2821	8.3	5.9	5.473	216.4	5.7	0.127231
8	5.554	230	28.3	0.0269434	5	0.227626	1.5	1	5.554	229	5	0.0254571
9	5.641	244	30	0.312439	4	2.24102	14.4	10.2	5.641	244.5	4.1	0.308659
10	5.734	259	31.8	0.0858932	8	1.10256	7.1	5	5.734	258.5	7.7	0.0804018
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.833	275	33.8	0.0472879	6	0.489438	3.1	2.2	5.833	275.7	5.7	0.0487327
12	5.894	284	34.9	0.197056	4	1.29833	8.4	5.9	5.894	284.1	3.9	0.189568
13	5.993	298	36.6	0.0256826	6	0.231272	1.5	1.1	5.993	300.3	5.6	0.0231013
14	6.287	340	41.8	0.0889687	7	1.10194	7.1	5	6.287	339.9	7.2	0.0869291
15	6.406	357	43.9	0.0422475	3	0.177591	1.1	0.8	6.406	357.1	2.8	0.0362142
16	7.127	458	56.3	0.10787	12	2.19022	14.1	10	7.127	458.2	11.6	0.106233

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	77	9.4	0.0148873	10	0.234573	10.7	7.6	4.55	76.2	9.7	0.0137063
2	5.2	173	21.2	0.0104046	12	0.179885	8.2	5.8	5.2	171.4	11.8	0.00858404
3	5.85	281	34.4	0.0163314	8	0.220166	10.1	7.1	5.85	279	8.3	0.0149496
4	6.55	382	46.8	0.0218278	9	0.644439	29.5	20.9	6.55	375.5	8.5	0.0426483
5	8.15	604	74	0.019297	10	0.329547	15.1	10.7	8.15	603.9	10.2	0.0181891
6	8.45	669	82	0.011416	12	0.202013	9.2	6.5	8.45	672.7	11.6	0.00985537
7	8.65	718	88	0.0115581	7	0.131463	6	4.3	8.65	718.4	7	0.0105626
8	9.3	787	96.4	0.0151685	10	0.244292	11.2	7.9	9.3	786.7	10.4	0.0132232

PeakCounter: 8

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 73

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 15.600 5.200 9.300 5.850 12.100 6.550 15.600 8.150 14.600 8.450 11.400 8.650 9.000 9.300 12.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 8 Return to Lane Navigator
4.246 1.100 4.322 1.600 4.423 31.300 5.108 0.600 5.248 1.100 5.435 11.000 5.512 4.700 5.589 0.100 5.686 11.400 5.763 3.000 5.834 4.700 5.896 7.400 6.001 0.200 6.291 7.400 6.408 1.100 7.136 13.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 16 Return to Lane Navigator
4.550 18.200 5.200 12.000 5.850 15.400 6.550 32.000 8.150 8.300 8.450 6.600 8.650 4.400 9.300 3.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 8 Return to Lane Navigator
4.311 1.200 4.416 1.900 4.521 24.400 5.126 0.800 5.200 0.500 5.258 1.000 5.457 12.200 5.553 5.800 5.732 11.300 5.816 2.100 5.904 4.000 5.968 6.200 6.096 0 6.337 11.900 6.444 3.600 6.550 5.000 7.199 8.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 17 Return to Lane Navigator
4.257 0.600 4.335 1.200 4.451 14.700 5.071 0.600 5.206 1.100 5.380 15.200 5.473 8.300 5.554 1.500 5.641 14.400 5.734 7.100 5.833 3.100 5.894 8.400 5.993 1.500 6.287 7.100 6.406 1.100 7.127 14.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 10.700 5.200 8.200 5.850 10.100 6.550 29.500 8.150 15.100 8.450 9.200 8.650 6.000 9.300 11.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 8 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: English Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.