Nitric oxide decreases intestinal haemorrhagic lesions in rat anaphylaxis independently of mast cell activation

doi:10.1080/09629359791893

Mediators Inflamm. 1997 February; 6(1): 25–31.

doi: 10.1080/09629359791893.

PMCID: PMC2365848

Nitric oxide decreases intestinal haemorrhagic lesions in rat anaphylaxis independently of mast cell activation

J. Carvalho Tavares, A. Moreno, and M. Sánchez Crespo

¹ Instituto de Biología y Geneética Molecular, CSIC-Facultad de Medicina, Valladolid, 47005, Spain,

M. Sánchez Crespo, Email: mscres/at/wamba.cpd.uva.es.

Corresponding author.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study is to assess the role of nitric oxide (NO) in the intestinal lesions of passive anaphylaxis, since this experimental model resembles necrotizing enterocolitis. Sprague-Dawley rats were sensitized with IgE anti-dinitrophenol monoclonal antibody. Extravasation of protein-rich plasma and haemorrhagia were measured in the small intestine. Plasma histamine was measured to assess mast cell activation. The effect of exogenous NO on the lesions was assessed by using two structurally unrelated NO-donors: sodium nitroprusside and S-nitroso-Nacetyl-penicillamine (SNAP). An increased basal production of NO was observed in cells taken after anaphylaxis, associated with a reduced response to platelet-activating factor, interleukin 1beta, and IgE/DNP-bovine serum albumin complexes. The response to bacterial lipopolysaccharide and dibutyryl cyclic adenosine monophosphate (AMP) was enhanced 24 h after challenge, but at earlier times was not significantly different from that observed in controls. Treatment with either sodium nitroprusside or SNAP produced a significant reduction of the haemorrhagic lesions, which are a hallmark of rat anaphylaxis. The extravasation of protein-rich plasma was not influenced by NO-donors. The increase of plasma histamine elicited by the anaphylactic challenge was not influenced by SNAP treatment. NO-donors protect intestinal haemorrhagic lesions of rat anaphylaxis by a mechanism apparently independent of mast cell histamine release.

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Selected References

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