RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Rougheye Rockfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes aleutianus
FDA Seafood List Common Name: Rougheye Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19980327gel04
Unique Web Code: reh01Srr
Tissue Aliquot Code: sebaaleu I94-001 #04m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	57	7.1	0.0891821	4	0.613767	8.3	5.8	4.55	57.4	3.8	0.0901123
2	5.2	156	19.4	0.0960636	4	0.720335	9.7	6.9	5.2	155.6	4.4	0.0927435
3	5.85	261	32.4	0.0812737	4	0.617963	8.3	5.9	5.85	260.6	4.3	0.0819997
4	6.55	398	49.4	0.151226	6	1.39154	18.8	13.2	6.55	397.3	5.7	0.138284
5	6.85	433	53.7	0.0345509	5	0.286693	3.9	2.7	6.85	432.9	5.1	0.0318879
6	7.35	501	62.2	0.0705529	4	0.508033	6.9	4.8	7.35	501.1	4.1	0.0707191
7	8.15	622	77.2	0.081217	8	1.12547	15.2	10.7	8.15	621.4	8.2	0.0775292
8	8.45	682	84.6	0.0995779	8	1.44076	19.4	13.7	8.45	680.9	8.5	0.0957096
9	8.65	734	91.1	0.0830903	5	0.706942	9.5	6.7	8.65	733.7	4.9	0.0806478

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.225	9	1.1	0.211585	5	1.94303	11	7.8	4.225	10.7	5.5	0.200318
2	4.451	41	5.1	0.125367	9	2.13041	12.1	8.6	4.451	42.5	9.4	0.128206
3	4.543	54	6.7	0.0523574	2	0.092381	0.5	0.4	4.543	53.2	1.9	0.0280531
4	4.599	62	7.7	0.0272377	1	0.0349528	0.2	0.1	4.599	62.5	1.4	0.0141266
5	4.691	75	9.3	0.161657	7	1.74547	9.9	7	4.691	77	6.9	0.14318
6	5.907	270	33.6	0.469506	4	3.61242	20.5	14.6	5.907	271	4.4	0.465448
7	6.003	289	35.9	0.216118	9	3.18959	18.1	12.9	6.003	287.7	8.9	0.201807
8	6.133	315	39.2	0.0833686	3	0.415139	2.4	1.7	6.133	314.8	2.8	0.0824825
9	6.178	324	40.3	0.0814716	4	0.628226	3.6	2.5	6.178	323.3	4.2	0.0841928
10	6.207	330	41	0.25792	2	0.892789	5.1	3.6	6.207	330.3	2	0.256947
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.343	357	44.4	0.126499	5	1.03839	5.9	4.2	6.343	356.5	4.8	0.122771
12	6.463	381	47.4	0.0905824	4	0.593066	3.4	2.4	6.463	380.5	3.8	0.0869792
13	6.503	389	48.4	0.0592711	4	0.427973	2.4	1.7	6.503	387.6	3.5	0.0681736
14	7.357	502	62.4	0.057718	2	0.244366	1.4	1	7.357	502.2	2.4	0.0584936
15	7.601	539	67	0.127614	3	0.665285	3.8	2.7	7.601	539.5	3	0.12467

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	50	6.2	0.08175	4	0.490892	6.8	4.6	4.55	50.7	3.5	0.0787602
2	5.2	135	16.8	0.0689981	5	0.618696	8.5	5.8	5.2	135.6	5.3	0.0656233
3	5.85	253	31.5	0.0810313	4	0.532985	7.3	5	5.85	253.4	3.8	0.0790675
4	6.55	396	49.3	0.139324	7	1.52758	21	14.3	6.55	395.4	6.7	0.129248
5	6.85	428	53.2	0.0416793	4	0.300229	4.1	2.8	6.85	428.5	4.3	0.0389977
6	7.35	498	61.9	0.0843578	4	0.511224	7	4.8	7.35	498	3.5	0.0822618
7	8.15	619	77	0.107057	7	1.20006	16.5	11.2	8.15	618.3	6.6	0.102042
8	8.45	676	84.1	0.112925	7	1.34353	18.5	12.5	8.45	675.5	6.9	0.109773
9	8.65	728	90.5	0.100695	4	0.746625	10.3	7	8.65	727.4	4.4	0.0954008

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.32	18	2.2	0.249137	7	2.89113	10.8	8	4.32	20	7.1	0.229673
2	4.537	51	6.3	0.183331	15	4.56635	17.1	12.6	4.537	50.9	15.1	0.170637
3	4.76	85	10.6	0.180865	7	1.0973	4.1	3	4.76	86	6.5	0.0946228
4	4.898	106	13.2	0.143174	22	5.80534	21.8	16	4.898	102.4	21.8	0.150584
5	5.2	152	18.9	0.0542305	3	0.220353	0.8	0.6	5.2	152.4	2.7	0.0454867
6	5.85	262	32.6	0.0119783	4	0.0842153	0.3	0.2	5.85	262.4	4.1	0.0116399
7	5.944	280	34.8	0.383094	5	3.37735	12.7	9.3	5.944	279.7	5.1	0.376778
8	6.032	297	36.9	0.200009	8	2.65616	10	7.3	6.032	295.6	7.5	0.19962
9	6.151	320	39.8	0.066079	3	0.342396	1.3	0.9	6.151	320.6	3	0.0653378
10	6.203	330	41	0.0718214	4	0.549144	2.1	1.5	6.203	329.2	4.2	0.0742982
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.235	336	41.8	0.18466	2	0.687528	2.6	1.9	6.235	336.6	2.1	0.187462
12	6.38	364	45.3	0.0963582	5	0.880268	3.3	2.4	6.38	363.5	5.3	0.0942331
13	6.505	388	48.3	0.0923099	12	1.81698	6.8	5	6.505	391.8	11.6	0.0884608
14	6.547	396	49.3	0.083386	2	0.0010742	0	0	6.547	397.8	2.1	0.0002874
15	6.85	434	54	0.0228455	7	0.237572	0.9	0.7	6.85	436	7	0.019223
16	7.35	505	62.8	0.0267487	4	0.186881	0.7	0.5	7.35	504	4.2	0.0251391
17	7.604	542	67.4	0.0675359	4	0.429867	1.6	1.2	7.604	542.4	3.5	0.0683535
18	8.45	688	85.6	0.0421618	6	0.466944	1.8	1.3	8.45	686.7	6.4	0.0413252
19	8.65	733	91.2	0.0530228	4	0.369913	1.4	1	8.65	732.8	4.1	0.0503171

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.27	13	1.6	0.172216	5	1.58354	8.7	4.9	4.27	14.2	5.4	0.165437
2	4.463	42	5.2	0.176796	6	1.74847	9.6	5.4	4.463	41.7	5.9	0.167891
3	4.497	47	5.8	0.158627	3	0.44917	2.5	1.4	4.497	47.8	3.1	0.0822997
4	4.543	54	6.7	0.0984729	3	0.561328	3.1	1.7	4.543	53.4	3.3	0.0948665
5	4.603	63	7.8	0.0448635	5	0.409549	2.2	1.3	4.603	60.9	4.5	0.0508136
6	4.697	77	9.6	0.174837	6	1.99987	11	6.2	4.697	79.6	6.5	0.174933
7	5.931	278	34.6	0.432239	5	3.87604	21.3	11.9	5.931	276.7	5.3	0.410513
8	6.01	293	36.4	0.19939	7	2.63255	14.5	8.1	6.01	293.4	7.4	0.2011
9	6.151	320	39.8	0.0572659	4	0.365985	2	1.1	6.151	319.5	3.8	0.0543933
10	6.198	329	40.9	0.0644065	5	0.573772	3.1	1.8	6.198	328.2	5.1	0.0634437
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.234	336	41.8	0.166845	3	1.04462	5.7	3.2	6.234	336.6	3.5	0.17012
12	6.391	366	45.5	0.0792716	6	0.826866	4.5	2.5	6.391	366	5.9	0.0791505
13	6.538	394	49	0.0752588	7	0.919432	5	2.8	6.538	394.2	7	0.0740894
14	6.586	401	49.9	0.0594025	7	0.348057	1.9	1.1	6.586	403.2	6.7	0.0294845
15	7.37	507	63.1	0.0234435	3	0.132795	0.7	0.4	7.37	507.7	3.2	0.0235039
16	7.626	545	67.8	0.0843626	5	0.745172	4.1	2.3	7.626	546.3	4.9	0.0865474

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	57	7.1	0.0942058	4	0.584214	8.7	5.9	4.55	56.9	3.6	0.091931
2	5.2	153	19.1	0.106172	4	0.717367	10.7	7.2	5.2	152.7	4	0.101145
3	5.85	263	32.8	0.0576446	5	0.511385	7.6	5.1	5.85	262.5	5	0.0575969
4	6.55	396	49.3	0.162246	5	1.33185	19.8	13.4	6.55	395.3	5.2	0.144638
5	6.85	436	54.3	0.0145442	6	0.201316	3	2	6.85	433.6	5.8	0.0194162
6	7.35	503	62.6	0.0493238	4	0.360808	5.4	3.6	7.35	502.8	4.2	0.0480442
7	8.15	624	77.7	0.0785154	9	1.22856	18.3	12.3	8.15	622.8	9.2	0.0750813
8	8.45	684	85.2	0.0810742	8	1.1608	17.3	11.7	8.45	683	8.2	0.0794359
9	8.65	733	91.3	0.0784226	5	0.629912	9.4	6.3	8.65	733.2	4.7	0.075322

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 77

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 8.300 5.200 9.700 5.850 8.300 6.550 18.800 6.850 3.900 7.350 6.900 8.150 15.200 8.450 19.400 8.650 9.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.225 11.000 4.451 12.100 4.543 0.500 4.599 0.200 4.691 9.900 5.907 20.500 6.003 18.100 6.133 2.400 6.178 3.600 6.207 5.100 6.343 5.900 6.463 3.400 6.503 2.400 7.357 1.400 7.601 3.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 15 Return to Lane Navigator
4.550 6.800 5.200 8.500 5.850 7.300 6.550 21.000 6.850 4.100 7.350 7.000 8.150 16.500 8.450 18.500 8.650 10.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.320 10.800 4.537 17.100 4.760 4.100 4.898 21.800 5.200 0.800 5.850 0.300 5.944 12.700 6.032 10.000 6.151 1.300 6.203 2.100 6.235 2.600 6.380 3.300 6.505 6.800 6.547 0 6.850 0.900 7.350 0.700 7.604 1.600 8.450 1.800 8.650 1.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 19 Return to Lane Navigator
4.270 8.700 4.463 9.600 4.497 2.500 4.543 3.100 4.603 2.200 4.697 11.000 5.931 21.300 6.010 14.500 6.151 2.000 6.198 3.100 6.234 5.700 6.391 4.500 6.538 5.000 6.586 1.900 7.370 0.700 7.626 4.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 8.700 5.200 10.700 5.850 7.600 6.550 19.800 6.850 3.000 7.350 5.400 8.150 18.300 8.450 17.300 8.650 9.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Rougheye Rockfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Rougheye Rockfish ---- Go to Display Gel Lane Navigator.