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Title Isolation, purification, and radiolabeling of a novel 120-kD surface protein on Blastomyces dermatitidis yeasts to detect antibody in infected patients
Creator/Author Klein, B.S. ; Jones, J.M.
Publication Date1990 Jan 01
OSTI IdentifierOSTI ID: 7244445
Other Number(s)ISSN0021-9738; CODEN: JCINA
Resource TypeJournal Article
Resource RelationJournal of Clinical Investigation ; Vol/Issue: 85:1
Subject550901 -- Pathology-- Tracer Techniques; FUNGAL DISEASES-- PATHOGENESIS;MEMBRANE PROTEINS-- RADIOIMMUNOASSAY; ANTIBODIES;ANTIGENS;CELL WALL;ELECTROPHORESIS;IODINE 125;LABELLING;MOLECULAR WEIGHT;SCANNING ELECTRON MICROSCOPY;YEASTS
Related SubjectBETA DECAY RADIOISOTOPES;BIOASSAY;CELL CONSTITUENTS;DAYS LIVING RADIOISOTOPES;DIAGNOSTIC TECHNIQUES;DISEASES;ELECTRON CAPTURE RADIOISOTOPES;ELECTRON MICROSCOPY;EUMYCOTA;FUNGI;IMMUNOASSAY;IMMUNOLOGY;INFECTIOUS DISEASES;INTERMEDIATE MASS NUCLEI;IODINE ISOTOPES;ISOTOPE APPLICATIONS;ISOTOPES;MICROORGANISMS;MICROSCOPY;NUCLEI;ODD-EVEN NUCLEI;ORGANIC COMPOUNDS;PLANTS;PROTEINS;RADIOASSAY;RADIOIMMUNODETECTION;RADIOIMMUNOLOGY;RADIOISOTOPES;TRACER TECHNIQUES
Description/Abstract No well-defined Blastomyces-specific antigens are currently available.^We used sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting to identify immunologically active molecules in the cell wall of B. dermatitidis.^A major immunoreactive 120-kD protein (WI-1) was present in all five strains studied and comprised 5% of the protein in the cell wall extract obtained after freezing and thawing yeast cells.^WI-1 was recognized by serum from all 10 patients with blastomycosis but by none of those from 5 patients with histoplasmosis.^It was purified by electroelution, radiolabeled with 125I, and incorporated into a radioimmunoassay (RIA) for serodiagnosis of blastomycosis.^Antibody to WI-1 was detected in 58 (85%) of 68 patients with blastomycosis (geometric mean titer, 1:2,981), in two (3%) of 73 patients with histoplasmosis, coccidioidomycosis, sporotrichosis, or candidiasis (titers, 1:86 and 1:91) and in none of 44 healthy persons.^WI-1 was shown to be a surface molecule abundant on B. dermatitidis yeasts that were indirectly stained with serum from a rabbit immunized with WI-1.^Approximately 0.93 pg of WI-1 or 4.7 x 10(6) WI-1 molecules were found on the surface of an individual yeast using an antigen-inhibition RIA; none was found on Histoplasma capsulatum or Candida albicans yeasts.^We conclude that WI-1 is a novel, immunologically active surface molecule on the invasive form of B. dermatitidis and that WI-1 can be used to reliably detect antibody and study the immunopathogenesis of blastomycosis.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 152-161
System Entry Date2001 May 13

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