CHESTER EM, SCALARONE G, SCALARONE GM; Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2002 Sep 27-30; 42: abstract no. M-204.
Idaho State University, Pocatello, ID
BACKGROUND: Our laboratory has been involved in the preparation and evaluation of yeast phase lysate antigens from various isolates of Blastomyces dermatitidis. This current study was performed to compare antigens with respect to the detection of antibody in immunized rabbits. METHODS:Serum specimens from rabbits immunized with 6 Blastomyces dermatitidis killed whole yeast cell preparations (isolates T-58: dog, Tennessee; T-27: polar bear, Tennessee; ERC-2: dog, Wisconsin; S: soil, Canada; ER-3: dog, Wisconsin; and 48938: bat, India) were assayed by an indirect peroxidase enzyme-linked immunosorbent assay (ELISA) for the detection of antibody. The rabbits were inoculated on days 0 and 45 and serum collected 7 days following the second administration of antigen. The sera were assayed using B. dermatitidis yeast phase lysate antigens prepared from the above isolates. RESULTS:The results indicated that all 6 whole yeast cell preparations stimulated antibody production with mean absorbance values ranging from 0.333 (ERC-2) to 0.131 (ER-3) when assayed with the lysate antigens. With regard to the detection of antibody with each individual lysate antigen against serum specimens produced by both the homologous and heterologous isolates, it was determined that the mean absorbance values ranged from 0.314 (T-58) to 0.190 (T-27). CONCLUSIONS: The T-58 yeast lysate antigen was the optimal reagent for detecting antibody, while three whole yeast cell antigens (T-58,ERC-2 and S) were optimal and comparable with respect to inducing an antibody response.
Publication Types:
Keywords:
- Animals
- Antibodies
- Antibodies, Fungal
- Antigens, Fungal
- Blastomyces
- Canada
- Dogs
- Enzyme-Linked Immunosorbent Assay
- Hemagglutination Tests
- Homicide
- Immune Sera
- India
- Rabbits
- Saccharomyces cerevisiae
- Serum
- Tennessee
- Ursidae
- Wisconsin
- Yeasts
- immunology
Other ID:
UI: 102267078
From Meeting Abstracts