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Avertin for Mouse Anesthesia

Stock Solution:
25g 2-, 2-, 2-Tribromoethanol (Aldrich T4, 840-2)
15.5ml tert-Amyl alcohol (Aldrich 24, 048-6)

Mix and warm to 40°C to dissolve solid. Do not heat. Tribromoethanol is light sensitive, so cover with foil. When completely dissolved wrap in foil and store at 4°C. If the tribromoethanol recrystallizes warm again to redissolve. Stock is good for many months.

To make final solution, mix 19.75ml Hanks salt solution with 0.25ml stock (can also use any growth medium, but phenol red in needed to monitor pH). The mixture will have to be gently warmed and stirred to dissolve the stock. Do not heat excessively as the alcohols will vaporize, and the mixture will not work. Heating should be the minimum required to achieve solution. If the mixture becomes acid (yellow), it has been heated too much and should be discarded. When dissolved store at 4°C. This working stock is good for up to 14 days.

To use, inject i.p. Dosage is 0.02ml/g body weight (a 25g mouse gets 0.5ml, a 30g mouse 0.6ml). Mice should go down in 2-3 mins and will remain asleep for 30-40 mins. Check for response to paw squeezing. If necessary administer extra anesthetic (.05 to .1 ml).



		Avertin for Mouse Anesthesia
	Stock Solution: 25g 2-, 2-, 2-Tribromoethanol (Aldrich T4, 840-2) 15.5ml tert-Amyl alcohol (Aldrich 24, 048-6) Mix and warm to 40°C to dissolve solid. Do not heat. Tribromoethanol is light sensitive, so cover with foil. When completely dissolved wrap in foil and store at 4°C. If the tribromoethanol recrystallizes warm again to redissolve. Stock is good for many months. To make final solution, mix 19.75ml Hanks salt solution with 0.25ml stock (can also use any growth medium, but phenol red in needed to monitor pH). The mixture will have to be gently warmed and stirred to dissolve the stock. Do not heat excessively as the alcohols will vaporize, and the mixture will not work. Heating should be the minimum required to achieve solution. If the mixture becomes acid (yellow), it has been heated too much and should be discarded. When dissolved store at 4°C. This working stock is good for up to 14 days. To use, inject i.p. Dosage is 0.02ml/g body weight (a 25g mouse gets 0.5ml, a 30g mouse 0.6ml). Mice should go down in 2-3 mins and will remain asleep for 30-40 mins. Check for response to paw squeezing. If necessary administer extra anesthetic (.05 to .1 ml).