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Title Kinetic studies of amylase and biomass production by Calvatia gigantea
Creator/Author Kekos, D. ; Macris, B.J.
Publication Date1987 Jan 01
OSTI IdentifierOSTI ID: 5564674
Other Number(s)CODEN: BIBIA
Resource TypeJournal Article
Resource RelationBiotechnol. Bioeng. ; Vol/Issue: 30:5
Research OrgNuclear Research Center ``DEMOCRITOS``, Aghia Paraskevi, Attiki 15 310, Greece. Biology Division
Subject140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989); AMYLASE-- BIOCHEMICAL REACTION KINETICS;SEEDS-- BIODEGRADATION;STARCH-- BIODEGRADATION;TANNIC ACID-- BIODEGRADATION; AGRICULTURAL WASTES;BENCH-SCALE EXPERIMENTS;FUNGI
Related SubjectAROMATICS;CARBOHYDRATES;CARBOXYLIC ACIDS;CHEMICAL REACTIONS;DECOMPOSITION;ENZYMES;GLYCOSYL HYDROLASES;HYDROLASES;HYDROXY COMPOUNDS;KINETICS;O-GLYCOSYL HYDROLASES;ORGANIC ACIDS;ORGANIC COMPOUNDS;ORGANIC WASTES;PHENOLS;PLANTS;POLYPHENOLS;POLYSACCHARIDES;REACTION KINETICS;REAGENTS;SACCHARIDES;WASTES
Description/Abstract Production of alpha-amylase (alpha-4, glucan 4-glucanohydrolase, EC 3.2.1.1) by microorganisms has been practiced for many years in small and large scale operations and the literature on this enzyme is voluminous.^Aspergillus niger and Aspergillus oryzae have been reported as the main fungal species used for commercial production of the enzyme.^On the other hand, large volumes of low-cost agricultural products such as acorn (the perisperm-free dry seed contains approximately 60% starch) are wasted in many countries and provide a challenge to biotechnology to efficiently utilize these rich sources of starch for the production of high added value products like enzymes.^C. gigantea is an edible puffball excreting high levels of alpha-amylase when cultivated on different sources of starch containing elevated quantities of toxic tannic compounds.^This fungus has been employed for the production of microbial protein from wastes and acorns containing high levels of toxic tannic compounds.^The same fungus was also reported to grow on both hydrolyzable and condensed tannins as sole carbon sources.^The present work was undertaken to investigate certain kinetic characteristics of alpha-amylase and biomass production by C. gigantea grown on soluble and acorn starch in a lab fermenter.^(Refs.^18).
Country of PublicationUnited States
LanguageEnglish
FormatPages: 681-684
System Entry Date2001 May 13

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