mar Robert *

S8ptemller 34, 1925.

     w811 1 wish that you were hers.  L?oan cem8 last m-day and is a8
enthusiastic as f am whidh is sasine; .a lot as you SOW me,   38 are getting
our laboratory m&q&d this week <md talldng over pl%m for work so that we
can begin mext -a+&.

   Doan is at the Sheldon, 49th .md Lexiq$on, a very attractive hotel which
v&s built originally especially for mm and BO ir particul:mly comfortable,  me$
take worn x101 38 we&l  RO Roan invited me to a dinner at the Cafeteria which
ia Out Of doorm on th8 16th f'bOr jUSt half my T&J.  There is a gyrnnamiu~~ md
a swi;na 1% pool and every kind of :I rest Iroorp you can th nk of, cards, pool,
mm romn eto.    l#J placre is not 80 swell.  I have three ro8m8, a living
and dini= room oombined, kitchmm bed room and bath.   f wanted more room, one
more bed room for guests but it would have been at the exoe1uB of living al1 the
tiaaa, with no aun and aith no outl88k except walls and my nsi@bom wa8hiag 80 I
oho+s the euxd.i&t far all the year around.   Doan is to get you a plr~ce at the
h8lton wh8n you come to lWw York, which we &are ho;$ng is going to b8 m&rely every
year au you promised,   After October my home addr8ss will b8 151 East '80th
Street, but trend all mail here at the Iasiti~te.

    Row aboat w&k.  Dr. Flexner came back this morning, lw very rested.
I havent had &any chance to talk about work yet but did break to him the id8a
that 3 wanted to do a little with tuberculosfd, to which he agreab, without
knowing the plen.   The proof og the sew Ch,art 12 came this morning .and is fine.
I would lik8 to keep it a few days wtil I have hpd a ohance to show it to
Dr. Flexner and will then send the rhole thiragy to paa, that is th0 wholcr-
du:?licate set of proofs of the charts whi& f now hold.    I am to go to
Baltimore Yriday ni@t of this week :znd have Atten to *~lacCruder to try to have
the proof of t&e colored pl:&&r xwdy for ma thnt &q.   Then I hmc ml tten to
Miss %dmker~e to try to have the page proof reedy for &a8, t?l8n 1 Wniidbe sure that
the gag8 reriererncee, are straight.   Vith cxtly ?&m StOUkSdal8 to read the page proof
I feel a little le:ay about it and so am going to ineiet on seeing it mgrself.

    Dom has called my attention to M&&mkin*s -p-per on The origin of the
mononwlear ohzgocytes of perltonea3 exudatgq, Amer. Jour. of Path, 1926, 1,
305-324.   It 8 the first volum of the r8organieed Jowpal of Medical Besear&
and if you haventt got it you ought to Send for 8 reprint.   R8 mot8 ustppppr8r
8vidently on the basis of those of ours in tkrs Wciety of Medicine and Biology and
than to his disczomforttW saw OUT t-88 13 the Came&e Sontributiomr before he
publirshed.   `Ilr8 effect fs a little aIrming.   H8 describti three ty 8s Of
gWeocytic mononu&eara,  n &&in8, a rosette xxd a non-rpsette.    The rosette
type by which h8 m8ans our monocyt8 of (3ours8 is ;?lain enough but he does not give e
anou& for us lx ba mare just whet ha does M%L   We rather thi& that he has
young and ild GlaWatO&'Zyt8S on his mix&d.   However his chisf point is that %t is
a?p%a%lly the lym-ih L'1xxdS, and in 2-.rti&&.r the sinuses of ths lgmh glands
that &1Y8 15~8 to his rW@bttr tv.   You se8 he ia intent on the endoth8lia;l
origin, so for the clasmatocyte he say@ v~~8ctiL~x andothelitcl? mcl for the rosette
type6 the lym?hatic endothelirqp,    His evidence is thirt  he inject8 a slat-
urated solution of neutral red into a lyqh &and fixes the tissue, cuts aectionr
with a technique that avoids alcohol, i;t.~,in# with metheleneb1u.o md finds that ther8
are m&y cells in the sinpees that whoa rosettes,


   He findq that only a few of the nuolai have stained with the neutral red
and he r6gards those as the few that have b86n dsmsged by the stain+   All th&t
is ~usaling to m6 beoau66 I am very sure that every single oells must have been
killed by the stain.   Of murm he co6rnent6 on our wt find.@ the roeetk
froms in the lym?h gland6.     I think that our observationa of last year on
the origin of the mono&es every where in the tisruer a2w very 8ound and
controvert the idea of an origin of them from the ly@@io endoth6litar&    &At
any rate, Doan and I both feel aa I rsmbmber that you did that our studier of
lymph glandr were very incomplete 60 the ifret st+P on our program is to
make many hnahrw of nonsal lm;lh gland6 and bwolas muoh m re femiliar with
the variation6 in the l@kh@laxad punotur66 th&n we now are.   Thlr we pl811 bo
with the id8a of teeting out ?&Gunk&W idea of ths origin of the monooyter.  To
that end we thought that we would study tuberaulzr lymph glands in the 681111,
way, by QUP tetohnique and al6a by %WpCrating hi&    s oonneotion with this
wr$~k we shall ks6p olosely in touch with K&ndw6ll  a
                                     to 6~18
t& thoraoia dtxat.                         just what he g8t8 from
             I am Vera eager about that end of it.

     2%~ seamd idea I have in oonnection with tuberrruldair 16 whir.   I hear
that goat@ etre immune to tubsroulorir.  I am going to find if that is rbally
k-66 and study the monooyter in go&a blood, ~g goat6 blood in tfrr;lu mallam
with referenus to tuberoulosir.   Its that that I want to con6ult Dr. Whit6 about
in ame it is really true.

   Our third plan ir the general ti6nts cniLtu$6 of the blood with referemo
to the &n$i&m&!yt&br seua that we talkedaboutla6tyear,

   Th6 neat, is an idea you will find in the paper that a further cheak on the
6lasnEbtoeyte-mano oyts idea oould b8 made by getting tuberoular lung6 Nbljh a6 we had
last year in ahronioally 6tain8d rabbit6 i.e. ohroniaally atCaned with
trypan bfu,.  In 6om6 of the rabbita lrmgr I did not 86s any olasmatooyter, 60 I
think that point ehould be follow6d w.    The more I think of it the tmre sum I
am that your cla6matoayts-monoayte disoovery la really important.   I thix& that it
is 6ound but that we mwt subjeot it to more t66tr.

   Then of cowwe we * to go on with Doan's e;elatinous mmrow Lsnd he hm gotten

hi6 biological matohiaa: of leuooaytss w&l under wa# and all ready for further work.

  I think that It was in Ida,hnkin~e paper that there was :L refer8noe to the faot
that in oluinoa pig@6 blood there were vary at~~piawus Kurloff bodies in the
lymphocytet.   You rexnamber that those thing w6 called p`arusiter were we finally
deoided in the lmhocytes not in the moaocyter.  I wonder if he lo right that th6y
are Xurloff bodisr.   I have the vagu66t ~risa about Kurloff bodies -and thought
that they acre framnts of nuulei., and at w rate thoso bodies in th6 guinea
pigs blood ::ere certainly not of. nuolear origin.    I& us know vhen you h3ve read
bWtuWn~$ paper vh-:t you think his t&roe 'Lg. es of xa~aa~lear cells aroe

   The next item in thi8 10~; letter I6 ~iy~3.   1 thh!: that 1 Wt8 to YOU
that he handed in a very long paper inuluding mu& that was old, or in w first paper
in thu 6~11 :Jemorkl V lumn sud thm hia two new 2ointr all arittan .aa if it ma all
new.  Dr. Streeter was glad to take hia work and publish it prov ded it was ~~81, 60
I -c;ent ~lver t.50 whole thing wita his c?;;d $kked him to vrite two short papers on hi6
two pointa.   He ha6 just written to me that he is so discouraged that h6 want6
to put the whole thiw off.   I have answorod that I s3n1 1 be in Baltimore next
Saturday and that we aan talk it all over, then I h:ive asked him to let me bring
the paper up here ;A& 701% on it a little wself.    His two Doints are, first
that those 01-s of thrombouytsr coma from the magaloUast8 t&t io from
endotheliurr~ seoond that the clas~xtocytos tlmt CXTE olxtsfde t370 veosel6 are directly
from mesenchyme not from endothelium on the soor@ that the ~X~CFWWUL~~ clump8


3

of cell8 in th8 chfc&S 4 to 5 clays old 80 not stain with the neutral red as much
as the endothelium itself.   I think f t a good point, the very thing that I
wanted studied.   He may be right and I $hlnk that we mnt every possible teet
of the olamatocyts-monoayte idea, iLmctional as wall as errbry logiaal.    1
shall try to get his p"aper md may bc a:118 to rend you a copy.   He ha only one
good clopy, so I think that I shall try to get a copy made here, and send him
bauk the original,

    Do let wa knon your pleas for work, hmr the start is made in tne teaching,
whether you have found a houso or not and every other bit of new%

     la regard to the ohedks for your two student8 at Nashville, we worked out
a system this mumor whiuh ought to aorlc fairlg pmptly.    1 have   voucher book
and AI&@ out the order and tear off one ha3f to send to Mm. &a&   she rsakaa old
the ahsder to the etudent and sends it tg him In aare of the fnstruator with whom he Is
working.   In that W~JT you will know whsn the uheok cxmer.    The voucher books
came to-day, the pagers are all in qy room so I can't send them off until to-mprrou
but they should get to Denver this we& and be sent off next week from llenbtw.  The
delays xi11 corn from two souses, first Lf Mrs. Swm hampens to be away and
seuondly the f'unde are kept pretty we&l invested #and th8 plan is to sell aecuritiem
when neoesrsary.  That i?w 3lWEbye t&e a few day&

Hope this won't bee too low to read,

Very cordSally youxs,