This set of web pages will take you through the process of performing a 3D reconstruction from single particle data. These pages will assume that you have a basic understanding of the principles of transmission electron microscopy (cryo or negative stain). Each page will typically contain a minimal outline of the steps you will need to perform, to provide a quick summary for advanced users. Click on one of these outline steps to get detailed instructions for that step. After the description, links to programs associated with this step are provided in parentheses).
Particle with 1 axis of rotational symmetry (eg - C3)
Particle with icosahedral symmetry
Last Modified: