EMAN

How to do a Reconstruction

This set of web pages will take you through the process of performing a 3D reconstruction from single particle data. These pages will assume that you have a basic understanding of the principles of transmission electron microscopy (cryo or negative stain). Each page will typically contain a minimal outline of the steps you will need to perform, to provide a quick summary for advanced users. Click on one of these outline steps to get detailed instructions for that step. After the description, links to programs associated with this step are provided in parentheses).
    Box particles from scanned micrographs or CCD frames (boxer, eman, other programs)

    When you begin doing a reconstruction, you will presumably start with a set of scanned micrographs or CCD images. The first step in doing a reconstruction is selecting the particles from the micrographs. This can be accomplished with boxer (or one of the other programs above). All of the micrographs must be scanned at the same resolution. The scan resolution should be at least 3 times smaller than the highest resolution you wish to obtain, ie - if you want a 9 Å reconstruction, your scanned images should be sampled at at least 3 Å/pixel.

    Select the type of particle you have data for:

    Particle with 1 axis of rotational symmetry (eg - C3)

    Asymmetric particle

    Particle with icosahedral symmetry

Last Modified: