EMAN

Refine 3d Model

Align and Average Particles

The set of particles within each class is iteratively aligned and averaged to create a class-average. One class is associated with each reprojection from the previous step. Each reprojection is expected to look similar, but not identical to the corresponding class-average generated in this step. The alignment is performed by aligning each particle to the reprojection. The aligned particles are then averaged together, possibly with CTF amplitude correction and Wiener filtration. This average then serves as a new alignment reference. This process allows the class average to diverge substantially from the reprojection. The process continues for a fixed number of iterations which generally approaches convergence.
 
 
 

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Last Modified: Sat Jan 23 02:33:54 1999