Invitrogen | Invitrogen Lux Primers



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Step 3 of 7: Design and Select Primers

Based on your target sequence and design parameters, the following labeled primers were generated. Place your mouse cursor on a primer's name to highlight its position in the target sequence. To select a labeled primer, click on the primer's name and its unlabeled counterparts will be displayed on the next page. Primers with the lowest Score best fit the design criteria (i.e., 0.0 is best). However, all primers within a score range of 0.0 - 4.0 are expected to perform within specifications.

Sort by: Strand, Score | Strand, Location | Location | Score

Name	Labeled Primer	Strand	3' Location	Score
klf5_449RL	gacgatTGCGCGTGTTTCAGATCGtC	Reverse	449	0.00
klf5_704RL	cacctgTTGTAGAGGCCAGTTCTCAGGtG	Reverse	704	0.00
klf5_721RL	ctacaaaCGCATGGTCTCTGGGATTTGtAG	Reverse	721	0.00
klf5_1306RL	cactctgTTGGCGAATTAACTGGCAGAGtG	Reverse	1306	0.00
klf5_1448RL	cacctgGCCCGTATGAGTCCTCAGGtG	Reverse	1448	0.00
klf5_1456RL	gactcatGGCTTCTCGCCCGTATGAGtC	Reverse	1456	0.00
klf5_1586RL	caacgGCGGGAGAAGCTGCGtTG	Reverse	1586	0.00
klf5_1634RL	caccagCTCGCTCGCTCAGTTCTGGtG	Reverse	1634	0.00
klf5_1538RL	cactacaCCCGTGTGCTTCCTGTAGtG	Reverse	1538	0.30
klf5_1129RL	caccatAAGAAACTGACTTGGCATGGtG	Reverse	1129	0.55
klf5_1242RL	cacctcTAGCGGCATAGGACGGAGGtG	Reverse	1242	0.64
klf5_1521FL	gaccgCTGGAGGTTTGCCCGGtC	Forward	1521	0.80
klf5_1673RL	gacgcGAGCGGACTGCAAGGCGtC	Reverse	1673	0.82
klf5_1622RL	cacatgCTGGTGGCGCTTCATGtG	Reverse	1622	1.00
klf5_1348RL	caacagTCCGGGTTACTCCTTCTGtTG	Reverse	1348	1.00
klf5_1126RL	gtacaccGAAACTGACTTGGCATGGTGtAC	Reverse	1126	1.17
klf5_700RL	cactcaAGGCCAGTTCTCAGGTGAGtG	Reverse	700	1.19
klf5_30FL	caacctaCAGGTACGCGCTCTCTTAGGtTG	Forward	30	1.20
klf5_649RL	cactgaaATGCTGTAAGGTATGCCTTCAGtG	Reverse	649	1.28
klf5_1607RL	gaccaGTGCAGCGCGAGGTGGtC	Reverse	1607	1.32
klf5_1009RL	gacaaacTCCATTACTGCCGTCTGGTTTGtC	Reverse	1009	1.45
klf5_1031RL	gacaccTGCCATAGAAACATTAAGGGTGtC	Reverse	1031	1.60
klf5_868RL	gaccaTGTGGTGGCGCTGTGGtC	Reverse	868	1.60
klf5_673RL	caacatgGGGAGGAAGACGTTCATGtTG	Reverse	673	1.78
klf5_1123RL	cacgtaACTGACTTGGCATGGTGTACGtG	Reverse	1123	1.80
klf5_1342FL	gacaggATTCGCCAACTCTCCCACCTGtC	Forward	1342	1.86
klf5_156RL	gatctcAAGGCGGGCACGGAGAtC	Reverse	156	2.00
klf5_452RL	gatctgGGTGCGCGTGTTTCAGAtC	Reverse	452	2.00
klf5_679RL	gaacgtGATGTCAGGGAGGAAGACGtTC	Reverse	679	2.00
klf5_795FL	gactcgTCAGCCACCAGAGCGAGtC	Forward	795	2.00
klf5_1011RL	caaaccTCCATTACTGCCGTCTGGTtTG	Reverse	1011	2.00
klf5_1586aRL	caacgGCGGGAGAAGCTGCGTtG	Reverse	1586	2.00
klf5_735RL	gaccatTTGATCTGTGTTACGCATGGtC	Reverse	735	2.05
klf5_1639RL	gaactgGCGCTCGCTCGCTCAGtTC	Reverse	1639	2.16
klf5_849RL	gtatcttGGTCTGGTGGGAGCTGAAGAtAC	Reverse	849	2.60
klf5_33FL	cagcaaTACGCGCTCTCTTAGGTTGCtG	Forward	33	2.60
klf5_1610RL	cacctcCATGTGCAGCGCGAGGtG	Reverse	1610	2.62
klf5_1326RL	caactctGTATCTGACAGGTGGGAGAGtTG	Reverse	1326	2.95
klf5_697RL	catcactGCCAGTTCTCAGGTGAGTGAtG	Reverse	697	3.00
klf5_1148FL	gaaactgCGTACACCATGCCAAGTCAGTtTC	Forward	1148	3.00
klf5_1348aRL	caacagTCCGGGTTACTCCTTCTGTtG	Reverse	1348	3.00
klf5_457RL	gaaacaGTGGTGGTGCGCGTGTtTC	Reverse	457	3.10
klf5_30aFL	caacctaCAGGTACGCGCTCTCTTAGGTtG	Forward	30	3.20
klf5_540FL	catcttgGAGGAGCTGGTCCAGACAAGAtG	Forward	540	3.60
klf5_673aRL	caacatgGGGAGGAAGACGTTCATGTtG	Reverse	673	3.78
klf5_1514FL	caaaccGGCTGCGACTGGAGGTtTG	Forward	1514	3.80
klf5_1263FL	gtagcgCACCTCCGTCCTATGCCGCtAC	Forward	1263	3.95
klf5_679aRL	gaacgtGATGTCAGGGAGGAAGACGTtC	Reverse	679	4.00
klf5_761RL	gaacctTGGCTGAAAATGGTAACAGGtTC	Reverse	761	4.00
klf5_1011aRL	caaaccTCCATTACTGCCGTCTGGTTtG	Reverse	1011	4.00
klf5_1079FL	cagcaACCCACACCCCTCTGCtG	Forward	1079	4.00
klf5_1370FL	gatccgCAACAGAAGGAGTAACCCGGAtC	Forward	1370	4.00
klf5_1605FL	gagcgCAACGCAGCTTCTCCCGCtC	Forward	1605	4.03
klf5_995RL	gacatgcGGTTTGTCGAACTGGGCATGtC	Reverse	995	4.05
klf5_1639aRL	gaactgGCGCTCGCTCGCTCAGTtC	Reverse	1639	4.16
klf5_1286FL	gaatcgTGCTTCCAAACTGGCGAtTC	Forward	1286	4.20
klf5_796RL	gacggAGGAGGAGGAGGGGCCGtC	Reverse	796	4.34
klf5_733RL	gagaccTGATCTGTGTTACGCATGGTCtC	Reverse	733	4.40
klf5_1348aFL	gtatctgCAACTCTCCCACCTGTCAGAtAC	Forward	1348	4.55
klf5_724RL	caaatcTACGCATGGTCTCTGGGATtTG	Reverse	724	4.60
klf5_111FL	cagccGGGCGGGAGAAAGTGGCtG	Forward	111	4.72
klf5_997RL	catgcGGTTTGTCGAACTGGGCAtG	Reverse	997	4.80
klf5_1231RL	gaatctACGGAGGTGGGGTGAGAtTC	Reverse	1231	4.80
klf5_1326aRL	caactctGTATCTGACAGGTGGGAGAGTtG	Reverse	1326	4.95
klf5_1148aFL	gaaactgCGTACACCATGCCAAGTCAGTTtC	Forward	1148	5.00
klf5_1682RL	cagtccAAGGATGGCAGAGCGGACtG	Reverse	1682	5.00
klf5_457aRL	gaaacaGTGGTGGTGCGCGTGTTtC	Reverse	457	5.10
klf5_929RL	gatcttcGGAAGGGACAGAGAGATGAAGAtC	Reverse	929	5.22
klf5_1082FL	gaacagcTAACCCACACCCCTCTGCTGtTC	Forward	1082	5.24
klf5_1387FL	gatacgGGATCTGGAGAAGCGACGTAtC	Forward	1387	5.40
klf5_1514aFL	caaaccGGCTGCGACTGGAGGTTtG	Forward	1514	5.80
klf5_721aFL	gaggcATCACTCACCTGAGAACTGGCCtC	Forward	721	6.00
klf5_761aRL	gaacctTGGCTGAAAATGGTAACAGGTtC	Reverse	761	6.00
klf5_1137RL	caagtcaCCTGCTGTGGAAGAAACTGACtTG	Reverse	1137	6.00
klf5_673aFL	gatgctACTGAAGGCATACCTTACAGCAtC	Forward	673	6.11
klf5_1286aFL	gaatcgTGCTTCCAAACTGGCGATtC	Forward	1286	6.20
klf5_1200RL	gaagtccTCTCAGCTTGTCTATCGGGACtTC	Reverse	1200	6.40
klf5_992RL	ctagacatTTGTCGAACTGGGCATGTCtAG	Reverse	992	6.60
klf5_738RL	catgcgCTGTCTTGATCTGTGTTACGCAtG	Reverse	738	6.62
klf5_1273RL	caaactgTGGGTTGTGAATCGCCAGTtTG	Reverse	1273	6.74
klf5_1231aRL	gaatctACGGAGGTGGGGTGAGATtC	Reverse	1231	6.80
klf5_530RL	cagacaaCTTCTCCATTTCACATCTTGTCtG	Reverse	530	6.84
klf5_1082aFL	gaacagcTAACCCACACCCCTCTGCTGTtC	Forward	1082	7.24
klf5_1157RL	caggcGGGGAAAGTAGGTGGCCtG	Reverse	1157	7.41
klf5_1114FL	catgccTCAATGAAACAGTTCCAGGGCAtG	Forward	1114	7.91
klf5_1579FL	catgcaAAGCCGTTCCAGTGCAtG	Forward	1579	7.96
klf5_1273aRL	caaactgTGGGTTGTGAATCGCCAGTTtG	Reverse	1273	8.74

Your Target Sequence

1	CGCGGGGCAGGTACGCGCTCTCTTAGGTTGCTGGTGGAGCTTGGGGGTGG	50
51	CGGGAGCGGGTTCCGGCGAGAGGGTGAGCGAGCGCTGGGGCGGGGCGGGA	100
101	GAAAGTGGCTGCCCGAGGACCTCGGCGGTGACGCGTGGCCGAGCCCAGGA	150
151	GCCCCGATCTCCGTGCCCGCCTTCGTGAGCGTCTGGCTGCCGGCCCAGGG	200
201	GTCCCCCGCCGCGGCCCCCCGCCGAGTCCGCCGTCCCGTGCCAGCCCGAG	250
251	CGAGGTGGGATCGCGATCGCTCCGTGTCCCGCTCCCGTAATCCCCAGACC	300
301	GTCCATGCCCACGCGGGTGCTGACCATGAGCGCCCGCCTGGGACCACTGC	350
351	CCCAGCCGCCGGCCGCGCAGGACGAGCCCGTGTTCGCGCAGCTCAAGCCG	400
401	GTGCTGGGCGCTGCGAACCCGGCCCGCGACGCGGCGCTCTTCTCCGGAGA	450
451	CGATCTGAAACACGCGCACCACCACCCGCCTGCGCCGCCGCCAGCCGCTG	500
501	GCCCGCGACTGCCCTCGGAGGAGCTGGTCCAGACAAGATGTGAAATGGAG	550
551	AAGTATCTGACCCCTCAGCTCCCTCCAGTTCCGATAATTTCAGAGCATAA	600
601	AAAGTATAGACGAGACAGTGCCTCAGTGGTAGACCAGTTCTTCACTGACA	650
651	CTGAAGGCATACCTTACAGCATCAACATGAACGTCTTCCTCCCTGACATC	700
701	ACTCACCTGAGAACTGGCCTCTACAAATCCCAGAGACCATGCGTAACACA	750
751	GATCAAGACAGAACCTGTTACCATTTTCAGCCACCAGAGCGAGTCGACGG	800
801	CCCCTCCTCCTCCTCCGGCCCCCACCCAGGCTCTCCCCGAGTTCACTAGT	850
851	ATCTTCAGCTCCCACCAGACCACAGCGCCACCACAGGAGGTGAACAATAT	900
901	CTTCATCAAACAAGAACTTCCTATACCAGATCTTCATCTCTCTGTCCCTT	950
951	CCCAGCAGGGCCACCTGTACCAGCTGTTGAATACACCGGATCTAGACATG	1000
1001	CCCAGTTCGACAAACCAGACGGCAGTAATGGACACCCTTAATGTTTCTAT	1050
1051	GGCAGGCCTTAACCCACACCCCTCTGCTGTTCCACAGACGTCAATGAAAC	1100
1101	AGTTCCAGGGCATGCCCCCTTGCACGTACACCATGCCAAGTCAGTTTCTT	1150
1151	CCACAGCAGGCCACCTACTTTCCCCCGTCACCACCAAGCTCAGAGCCTGG	1200
1201	AAGTCCCGATAGACAAGCTGAGATGCTGCAGAATCTCACCCCACCTCCGT	1250
1251	CCTATGCCGCTACAATTGCTTCCAAACTGGCGATTCACAACCCAAATTTA	1300
1301	CCTGCCACTCTGCCAGTTAATTCGCCAACTCTCCCACCTGTCAGATACAA	1350
1351	CAGAAGGAGTAACCCGGATCTGGAGAAGCGACGTATCCACTTCTGCGATT	1400
1401	ATAATGGTTGCACAAAAGTTTATACAAAGTCGTCTCACTTAAAAGCTCAC	1450
1451	CTGAGGACTCATACGGGCGAGAAGCCCTACAAGTGCACCTGGGAGGGCTG	1500
1501	CGACTGGAGGTTTGCCCGGTCGGATGAGCTGACCCGCCACTACAGGAAGC	1550
1551	ACACGGGCGCCAAGCCGTTCCAGTGCATGGTGTGCCAACGCAGCTTCTCC	1600
1601	CGCTCCGACCACCTCGCGCTGCACATGAAGCGCCACCAGAACTGAGCGAG	1650
1651	CGAGCGCTGCGCCCACCCGCCTGACGCCTTGCAGTCCGCTCTGCCATCCT	1700
1701	TTAAACCGCAGACCTAACTTCATAAAAAGAAAAAAAAA	1750

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